Data is not available to indicate whether DNA contamination could affect sequencing. However, including DNase treatment is recommended to minimize genomic DNA in the sequencing reads.
STRN250
Spectrum™ Plant Total RNA Kit
sufficient for 250 purifications
Synonym(s):
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
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About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
Pricing and availability is not currently available.
usage
sufficient for 250 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
RNA purification: suitable
test parameters
: ~30 min kit run time, sample volume: 100 mg
greener alternative category
storage temp.
15-25°C
General description
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.
Application
Spectrum™Plant Total RNA Kit has been used:
- to extract RNA from the material ground in liquid nitrogen
- to isolate total RNA from mutant and wt (wild type) plantlets
- to extract total RNA from leaf samples
- to extract RNA from all plant tissues
Features and Benefits
- Yields up to 60 μg of pure concentrated RNA per prep
- Efficient protocol allows for RNA purification in 30 min or less
- Specially designed for research with difficult plant tissues
Legal Information
Spectrum is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable |
| usage sufficient for 250 purifications | usage sufficient for 50 purifications | usage sufficient for 10 purifications | usage sufficient for 350 purifications |
| greener alternative category | greener alternative category | greener alternative category , Aligned | greener alternative category |
| test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters : ~30 min kit run time, sample volume: 100 mg | test parameters : <30 min kit run time, sample volume: up to 10^7 cells or 40 mg of tissue |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Kit Components Only
Product No.
Description
- Binding Solution
- Lysis Solution
- Wash Solution 1
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
- 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) 2
signalword
Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
target_organs
Liver,Heart
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
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I’m using your plant RNA extraction kit for RNA-seq. If I skip the DNase treatment, could DNA contamination affect the sequencing library, or is it mandatory to include this step? Thank you.
1 answer-
Helpful?
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does it sufficient for small RNA and/or non-coding RNA extraction?
1 answer-
Yes, this kit is sufficient for small RNA. However, It is worth noting that small RNA molecules, such as microRNA, siRNA, tRNA, 5S rRNA, are not efficiently recovered using the standard procedure. If desired, these small RNA molecules can be recovered by increasing the amount of binding solution, as described in Protocol A under Procedure Step 4.
For more information, please see the protocol associated with this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/323/177/strn250bul.pdfHelpful?
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