P6282

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Synonym(s): PLL HBr
• CAS Number: 25988-63-0
• UNSPSC Code: 12352202
• eCl@ss: 32160406
• PubChem Substance ID: 24898753
• NACRES: NA.75
• MDL number: MFCD00237230
• Biological source: synthetic (organic)
• Form: lyophilized powder
• Technique(s): cell culture | mammalian: suitable
• Mol wt: 70,000-150,000

Properties

• Product Name: Poly-L-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• biological source: synthetic (organic)
• Quality Level: 200
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• mol wt: 70,000-150,000
• packaging: pkg of 5 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 4 μg/cm²
• solubility: H₂O: soluble 50 mL/vial, clear, colorless
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• InChI key: WBSCNDJQPKSPII-KKUMJFAQSA-N

Description

General description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.

Biochem/physiol Actions

Poly-L-lysine serves as a pre-coating substance to ensure better attachment of cells to culture surfaces.[1] Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Disclaimer

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)