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α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Name: α(2→3,6,8,9) Neuraminidase from <I>Arthrobacter ureafaciens</I>
Brand: SIGMA

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(s):

Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase

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About This Item

CAS Number:

9001-67-6

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.2.1.18 (BRENDA, IUBMB)

MDL number:

MFCD00131711

Specific activity:

≥135 units/mg protein

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Segment

200

form

buffered aqueous solution

specific activity

≥135 units/mg protein

mol wt

88 kDa, 95 kDa

foreign activity

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Description

Biochem/physiol Actions

Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.

Preparation Note

Expressed in glycosidase-free hosts.

Other Notes

One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

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1 of 1

This Item	N7271	480716	N3001
Sigma-Aldrich N8271 α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens	Sigma-Aldrich N7271 α(2→3) Neuraminidase from Streptococcus pneumoniae	Sigma-Aldrich 480716 α2-3,6,8,9-Neuraminidase, Arthrobacter ureafaciens, Recombinant, E. coli	Sigma-Aldrich N3001 Neuraminidase from Clostridium perfringens (C. welchii)
specific activity ≥135 units/mg protein	specific activity -	specific activity ≥40 units/mg protein, ≥5 units/mL	specific activity ≥2 units/mg protein (mucin), ≥6 units/mg protein (using 4MU-NANA)
form buffered aqueous solution	form buffered aqueous solution	form liquid	form lyophilized powder
recombinant expressed in E. coli	recombinant -	recombinant expressed in E. coli	recombinant -
mol wt 88 kDa, 95 kDa	mol wt -	mol wt -	mol wt -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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