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H6533
Anti-HA−Peroxidase antibody, Mouse monoclonal
clone HA-7, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
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About This Item
Conjugate:
peroxidase conjugate
Clone:
HA-7, monoclonal
Application:
WB
Citations:
135
Pricing and availability is not currently available.
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
concentration
5-11 mg/mL
technique(s)
western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent
storage temp.
2-8°C
General description
Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains.
The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Application
Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used:
- in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells[1]
- in immunoblot detection in transfected A375 cells
- is suitable for immunoblotting of nucleosome assembly protein NAP1L1
Biochem/physiol Actions
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Reconstitute in .5mL of distilled water.
1 of 1
This Item | |||
|---|---|---|---|
| clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal | clone HIS-1, monoclonal |
| conjugate peroxidase conjugate | conjugate alkaline phosphatase conjugate | conjugate TRITC conjugate | conjugate alkaline phosphatase conjugate |
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| technique(s) western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent | technique(s) western blot: 1:4,000 using extracts of mammalian cells expressing HA tagged fusion proteins | technique(s) direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins | technique(s) western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein |
| form lyophilized powder | form buffered aqueous glycerol solution | form buffered aqueous solution | form buffered aqueous glycerol solution |
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| H6533-1VL | 04061833801475 |
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What is the Department of Transportation shipping information for this product?
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
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Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
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