E8640

E-64d

powder, ≥98% (TLC)

• Synonym(s): (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester, EST
• Empirical Formula (Hill Notation): C₁₇H₃₀N₂O₅
• CAS Number: 88321-09-9
• Molecular Weight: 342.43
• UNSPSC Code: 12352202
• PubChem Substance ID: 24894681
• NACRES: NA.77
• MDL number: MFCD00132883

Properties

• Product Name: E-64d, protease inhibitor
• biological source: synthetic (organic)
• Quality Segment: 200
• assay: ≥98% (TLC)
• form: powder
• solubility: methanol: water (1:1): 5 mg/mL, clear, colorless
• storage temp.: −20°C
• SMILES string: CCOC(=O)[C@H]1O[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)NCCC(C)C
• InChI: 1S/C17H30N2O5/c1-6-23-17(22)14-13(24-14)16(21)19-12(9-11(4)5)15(20)18-8-7-10(2)3/h10-14H,6-9H2,1-5H3,(H,18,20)(H,19,21)/t12-,13-,14-/m0/s1
• InChI key: SRVFFFJZQVENJC-IHRRRGAJSA-N
• Gene Information: human ... CAPN1(823)

Description

Application

E-64d has been used as a lysosomal inhibitor in human acute promyelocytic leukemia NB4 cells and Huh-7 cells. It has also been used as a protease inhibitor in free calcium physiological tyrode solution for perfusion into the isolated heart samples.

Biochem/physiol Actions

E-64d is an epoxysuccinyl peptide and an inhibitor of cysteine protease cathepsin B, calpains 1 and 2. E-64d by its cathepsin B protease inhibition functionality, may serve as a potential drug for treating traumatic brain injury (TBI) and Alzheimer′s disease (AD). It inhibits gametocyte surface antigen resulting in a decreased oocyst production in Plasmodium falciparum.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves