For assay purposes, this material is prepared in cold deionized water without the addition of polyhexamethylene biguanide. Stock solutions may be further diluted in 50 mM sodium acetate buffer, pH 5.0. The addition of polyhexamethylene biguanide (PHMB) is not required. Please see the link below to review additional information available in the enzyme assay protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/352/413/c8546enz.pdf
C8546
Cellulase from Trichoderma reesei ATCC 26921
lyophilized powder, ≥1 unit/mg solid
Synonym(s):
1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
eCl@ss:
32160410
Specific activity:
≥1 unit/mg solid
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
specific activity
≥1 unit/mg solid
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
solubility
deionized water: soluble 5.0 mg/mL
greener alternative category
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Application
Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose; to study the biodegradability of bioabsorbable bacterial cellulose (BBC) material.[1]
Cellulase from Trichoderma reesei has been used in a study to assess the differential regulation of the cellulase transcription factors XYR1, ACE2, and ACE. Cellulase from Trichoderma reesei has also been used in a study to investigate the liquefaction of hydrothermally pretreated wheat straw at high-solids content.
Biochem/physiol Actions
Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries. Cellulase is added to detergents to improve cleansing properties.[2] It is also applied in the biodegradation of paper products such as foolscap paper, filter paper, newspaper and office paper as well as microcrystalline cellulose.[3]
Preparation Note
Dissolves in sterile deionized (DI) water in the presence of 0.15% polyhexamethylene biguanide (PHMB) at 5 mg/mL concentration.
Other Notes
One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| specific activity ≥1 unit/mg solid | specific activity ≥5,000 units/g solid | specific activity 3-10 units/mg solid | specific activity ≥700 units/g |
| form lyophilized powder | form powder | form powder | form aqueous solution |
| solubility deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).) | solubility deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB)) | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| greener alternative category | greener alternative category | greener alternative category , Enabling | greener alternative category |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| C8546-10KU | 04061833522424 |
| C8546-2.5KU | 04061833522431 |
| C8546-5KU | 04061833522448 |
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Do you know how the solubility and activity of the cellulase is affected if you do not add PHMB?
1 answer-
Helpful?
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Do you know the activity versus pH response ?
1 answer-
The optimal conditions for this product are 37°C and pH 5.0. According to literature, cellulase activity decreases when the pH is higher or lower than the range of 5.0 to 6.0.
Helpful?
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How has this enzyme been purified, Does it have a His-tag?
1 answer-
This product is a partially purified, diafiltered, lyophilized cellulase complex containing exo- and endoglucanase activities. It is a native protein and does not include a His-tag.
Helpful?
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