B8931

Magenta-Gal

reagent for selection of recombinant bacterial clones

• Synonym(s): Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside
• Empirical Formula (Hill Notation): C₁₄H₁₅BrClNO₆
• CAS Number: 93863-88-8
• Molecular Weight: 408.63
• NACRES: NA.85
• PubChem Substance ID: 329773145
• UNSPSC Code: 12352200
• MDL number: MFCD02683897

Properties

• grade: Molecular Biology
• sterility: non-sterile
• product line: BioReagent
• assay: ≥98.0% (HPLC)
• form: powder
• solubility: methanol: soluble
• suitability: suitable for substrate for β-galactosidase
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1
• InChI key: CHRVKCMQIZYLNM-HTOAHKCRSA-N

Description

General description

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Red-Gal is a chromogenic substrate for b-galactosidase,[1] used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.

Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein.[1] ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.

Biochem/physiol Actions

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)