A9576 is prepared with modified DPBS without calcium chloride and magnesium chloride.
A9576
Bovine Serum Albumin solution
30% in DPBS, sterile-filtered, BioXtra, suitable for cell culture
Bovine Serum Albumin solution
Synonym(s):
Albumin solution from bovine serum, BSA solution
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About This Item
Biological source:
bovine
Assay:
28-32% total protein basis (UV)
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Impurities:
endotoxin, tested
Concentration:
in phosphate buffer (DPBS)
Pricing and availability is not currently available.
biological source
bovine
Quality Level
sterility
sterile-filtered
product line
BioXtra
assay
28-32% total protein basis (UV)
form
liquid
origin
USA origin
concentration
in phosphate buffer (DPBS)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
6.5-7.5
foreign activity
Mycoplasma Test by Barile on Raw material, none detected
shipped in
ambient
storage temp.
2-8°C
General description
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Application
BSA 30% solution is a convenient form for direct supplementation of serum-free media. Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Preparation Note
Prepared from A9647 in DPBS (D5652).
1 of 1
This Item | |||
|---|---|---|---|
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| technique(s) cell culture | mammalian: suitable | technique(s) blocking: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| assay 28-32% total protein basis (UV) | assay 33-37% total protein basis (UV) | assay 90-120 mg/mL protein basis (biuret) | assay ≥96% (agarose gel electrophoresis) |
| Quality Level 300 | Quality Level 400 | Quality Level 300 | Quality Level 300 |
| form liquid | form liquid | form solution | form lyophilized powder |
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
J A A Pires et al.
Journal of dairy science, 102(6), 5347-5360 (2019-03-25)
The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows. Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for
Paul W Kriebel et al.
The Journal of cell biology, 217(8), 2891-2910 (2018-06-10)
Chemotactic signals are relayed to neighboring cells through the secretion of additional chemoattractants. We previously showed in Dictyostelium discoideum that the adenylyl cyclase A, which synthesizes the chemoattractant cyclic adenosine monophosphate (cAMP), is present in the intraluminal vesicles of multivesicular
Yasuhiro Kawai et al.
PloS one, 13(5), e0196655-e0196655 (2018-05-26)
Serum albumin is the major intravascular antioxidant. Though oxidative stress plays an important role in the pathophysiology of Immunoglobulin A nephropathy (IgAN), the association between serum albumin and the progression of IgAN is not entirely understood. This retrospective cohort study
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9576-50ML | 04061838259097 |
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Is this product prepared in DPBS with or without Ca++ and Mg++?
1 answer-
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What is the open stability of A9576?
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This product is assigned a recommended retest date of approximately 3 years from the date of Quality Release. Provided that the product is stored properly and no contaminants have been introduced, once opened, the product is expected to be stable up to the lot-specific retest date. The retest date is published in the product Certificate of Analysis. Please see the link below to access a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a9576#product-documentationHelpful?
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