A0731

Anti-ATG5 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Atg5 (C-terminal) antibody produced in rabbit, Anti-ASP, Anti-Apg5, Anti-Apg5L, Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD09265351
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 67

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen ~56 kDa
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: suitable, western blot: 1-2 μg/mL using whole extract of mouse liver or human U87 cells
• UniProt accession no.: Q9H1Y0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ATG5(9474) ; mouse ... Atg5(11793)

Description

General description

ATG5 (autophagy related 5) is located on human chromosome band 6q21.

ATG5 was found localized to double FYVE domain containing protein 1 (DFCP1)- specific omegasomes. [1]

Macroautophagy, usually referred to as autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. Autophagy is an evolutionary conserved pathway seen in all eukaryotic cells. At least 16 ATG genes, required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals. Atg5, also known as Apg5, is a 32 kDa protein essential for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. This conjugation reaction requires ATP and two enzymes, Atg7 and Atg10, which are E1- and E2-like enzymes, respectively. The Atg12-Atg5 conjugate interacts non-covalently with Atg16. The Atg12-Atg5-Atg16 complex localizes to autophagosome precursors and plays an essential role in autophagosome formation. It has been reported that in addition to the role of Atg5 in the formation of autophagosomes, an Atg5 fragment produced by calpain cleavage has pro-apoptotic properties.

Rabbit polyclonal anti-ATG5 (C-terminal) antibody recognizes mouse and human Atg5-Atg12 complex by immunoblotting, ~56 kDa. Detection of the Atg5-Atg12 complex band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 262-275 of mouse Atg5, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by one amino acid in human.

Application

Anti-ATG5 (C-terminal) antibody has been used in immunohistochemical (IHC) staining.

Rabbit anti-ATG5 (C-terminal) antibody has been used for western blot analysis in U2OS cells. The antibody has also been used for immunoblot assays in CT26 and MCA205 cells.

Rabbit polyclonal anti-ATG5 (C-terminal) antibody is used to tag Atg5-Atg12 complex for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Atg5-Atg12 complex in the formation and function of autophagosomes. Rabbit anti-ATG5 was used as primary antibody for immunocytochemistry on HEK293 and Cos7 cells. Cells were fixed in 3.7% formaldehyde in 200 mM Hepes prior to antibody treatment.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)