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43819

DL-Dithiothreitol

≥99.0% (RT)

Synonym(s):

threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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About This Item

Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS Number:
3483-12-3
Molecular Weight:
154.25
UNSPSC Code:
12352201
NACRES:
NA.21
PubChem Substance ID:
329756409
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:
MFCD00004877
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Quality Segment

200

assay

≥99.0% (RT)

form

powder

reaction suitability

reagent type: reductant

impurities

≤0.3% 4,5-dihydroxy-1,2-dithiane

mp

40-43 °C, 41-44 °C (lit.)

solubility

H2O: 50 mg/mL, clear, colorless, H2O: freely soluble, acetone: freely soluble, chloroform: freely soluble, diethyl ether: freely soluble, ethanol: freely soluble, ethyl acetate: freely soluble

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤250 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
DL-Dithiothreitol is a pivotal component used in the preparation of buffer which in turn is utilized in the platelet cytosol preparation.[1] It is also important in isolation of RNA by using guanidinium salts and homogenisation of tissue[2] and cells[3].

Biochem/physiol Actions

DL-Dithiothreitol denatures proteins by reducing the disulphide bonds and reducing them to SH groups. It also prevents intramolecular and intermolecular disulfide bond formation between cysteine residues of proteins.[4]

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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This Item
D0632D9163D9779
DL-Dithiothreitol ≥99.0% (RT)

Sigma-Aldrich

43819

DL-Dithiothreitol

DL-Dithiothreitol ≥98% (HPLC), ≥99.0% (titration)

Sigma-Aldrich

D0632

DL-Dithiothreitol

DL-Dithiothreitol suitable for electrophoresis, ≥99%

Sigma-Aldrich

D9163

DL-Dithiothreitol

DL-Dithiothreitol Molecular Biology, ≥98% (HPLC), ≥99% (titration)

Sigma-Aldrich

D9779

DL-Dithiothreitol

assay

≥99.0% (RT)

assay

≥98% (HPLC), ≥99.0% (titration)

assay

≥99%

assay

≥98% (HPLC), ≥99% (titration)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

solubility

H2O: 50 mg/mL, clear, colorless, acetone: freely soluble, diethyl ether: freely soluble, ethyl acetate: freely soluble, H2O: freely soluble, ethanol: freely soluble, chloroform: freely soluble

solubility

H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 50 mg/mL, clear, colorless to very faintly yellow

solubility

H2O: 50 mg/mL

form

powder

form

powder

form

powder

form

powder

mp

40-43 °C, 41-44 °C (lit.)

mp

41-44 °C (lit.)

mp

40-44 °C, 41-44 °C (lit.)

mp

41-44 °C (lit.)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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