Key Documents

Vimentin (V9) Mouse Monoclonal Antibody

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

V9, monoclonal

Application:

IHC (p)

Citations:

Pricing and availability is not currently available.

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Properties

biological source

mouse

Quality Level

100, 500

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

V9, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (347M-14), vial of 0.5 mL concentrate (347M-15), bottle of 1.0 mL predilute (347M-17), vial of 1.0 mL concentrate (347M-16), bottle of 7.0 mL predilute (347M-18)

manufacturer/tradename

Cell Marque^®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500

isotype

IgGκ

control

tonsil

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic

Gene Information

human ... VIM(7431)

Description

General description

Anti-vimentin is of limited value as a diagnostic tool; however, when used in combination with other antibodies (in panels) it is useful for the subclassification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. This antibody recognizes a 57 kD intermediate filament. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Analysis Note

IVD

RUO

Other Notes

For Technical Service please contact: 800-665-7284 or email: [email protected]

Vimentin Positive Control Slides, Product No. 347S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

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Show Differences

1 of 1

This Item	MAB3400	SAB5500191	V6389
Sigma-Aldrich 347M-1 Vimentin (V9) Mouse Monoclonal Antibody	Sigma-Aldrich MAB3400 Anti-Vimentin Antibody, clone V9	Sigma-Aldrich SAB5500191 Anti-Vimentin antibody, Rabbit monoclonal	Sigma-Aldrich V6389 Anti-Vimentin antibody, Mouse monoclonal
biological source mouse	biological source mouse	biological source rabbit	biological source mouse
clone V9, monoclonal	clone V9, monoclonal	clone SP20, monoclonal	clone V9, monoclonal
antibody form ascites fluid	antibody form purified antibody	antibody form tissue culture supernatant	antibody form purified from hybridoma cell culture
conjugate unconjugated	conjugate -	conjugate unconjugated	conjugate unconjugated
species reactivity human	species reactivity monkey, feline, pig, bovine, rat, human, chicken, canine	species reactivity human (tested)	species reactivity hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human
Gene Information human ... VIM(7431)	Gene Information human ... VIM(7431)	Gene Information human ... VIM(7431)	Gene Information human ... VIM(7431) rat ... Vim(81818)

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

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Differential control of cytokeratins and vimentin synthesis by cell-cell contact and cell spreading in cultured epithelial cells.

A Ben-Ze'ev

The Journal of cell biology, 99(4 Pt 1), 1424-1433 (1984-10-01)

The expression of cytokeratins and vimentin was investigated in Madin-Darby bovine epithelial cells (MDBK) in culture under conditions of varied cell spreading and cell-cell contact. When extensive cell-cell contact was achieved by seeding cells at high density in monolayer, or

Vimentin: an evaluation of its role as a tumour marker.

M Leader et al.

Histopathology, 11(1), 63-72 (1987-01-01)

In this study we examined 198 sarcomas, 38 carcinomas, 13 'tumours with a spindle cell component' and 22 malignant melanomas with a commercial monoclonal vimentin antibody. All histopathological material was formalin fixed and paraffin embedded. The results show this antibody

Immunophenotyping of non-Hodgkin's lymphomas using a panel of antibodies on paraffin-embedded tissues.

F R Davey et al.

The American journal of pathology, 129(1), 54-63 (1987-10-01)

The use of monoclonal and polyclonal antibodies for the immunophenotyping of non-Hodgkin's lymphomas in paraffin-embedded tissue has been limited by the fact that most antigens on lymphoid cells are denatured by histologic fixation, dehydration, and embedment. In this article the

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Global Trade Item Number

SKU	GTIN
347M-16	0841683137672
347M-17	0841683137689
347M-15	0841683137665
347M-18	0841683137696
347M-14	0841683137658

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