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97622

2-Mercaptoethanol

derivatization grade (HPLC), LiChropur™, ≥99.0% (GC)

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
UNSPSC Code:
23151817
NACRES:
NA.05
PubChem Substance ID:
329770550
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
MFCD00004890
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grade

derivatization grade (HPLC)

Quality Level

100

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

assay

≥99.0% (GC)

form

liquid

quality

LiChropur™

expl. lim.

18 %

concentration

14.3 M (pure liquid)

technique(s)

HPLC: suitable

refractive index

n20/D 1.500 (lit.), n20/D 1.500-1.502

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

General description

2-Mercaptoethanol is a thiol compound showing growth promoting properties on mouse lymphoma L1210 cells in vitro. It also enhances viability, blast transformation and antibody formation in lymphocyte cultures.

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
suitable for derivatisation of Amine groups

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

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This Item
PHR9274M6250M3148
2-Mercaptoethanol derivatization grade (HPLC), LiChropur™, ≥99.0% (GC)

Supelco

97622

2-Mercaptoethanol

2-Mercaptoethanol certified reference material, TraceCERT®

Supelco

PHR9274

2-Mercaptoethanol

2-Mercaptoethanol ≥99.0%

Sigma-Aldrich

M6250

2-Mercaptoethanol

2-Mercaptoethanol Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Sigma-Aldrich

M3148

2-Mercaptoethanol

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

grade

derivatization grade (HPLC)

grade

certified reference material, TraceCERT®

grade

-

grade

Molecular Biology

assay

≥99.0% (GC)

assay

-

assay

≥99.0%

assay

99% (GC/titration)

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

form

liquid

form

-

form

liquid

form

liquid

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Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN7818
BCCN2095
BCCM7502
BCCL6859
BCCK0471

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Mechanism of growth stimulation of L1210 cells by 2-mercaptoethanol in vitro. Role of the mixed disulfide of 2-mercaptoethanol and cysteine
Ishii T, et al.
The Journal of Biological Chemistry, 256(23), 12387-12392 (1981)
Katharina Rothe et al.
Blood, 123(23), 3622-3634 (2014-04-24)
Previous studies demonstrated that imatinib mesylate (IM) induces autophagy in chronic myeloid leukemia (CML) and that this process is critical to cell survival upon therapy. However, it is not known if the autophagic process differs at basal levels between CML
Leon Tribolet et al.
The Journal of infectious diseases, 211(3), 416-425 (2014-08-21)
Na-ASP-2 is an efficacious hookworm vaccine antigen. However, despite elucidation of its crystal structure and studies addressing its immunobiology, the function of Na-ASP-2 has remained elusive. We probed a 9000-protein human proteome microarray with Na-ASP-2 and showed binding to CD79A
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Global Trade Item Number

SKUGTIN
97622-1ML-KC04061838089250
97622-10X1ML04061833325087
97622-10ML04061833325070
97622-1ML04061838202598

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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