MABF106

Anti-Viperin Antibody, clone MaP.VIP

clone MaP.VIP, from mouse

• Synonym(s): Radical S-adenosyl methionine domain-containing protein 2, Cytomegalovirus-induced gene 5 protein, Viperin, Virus inhibitory protein,endoplasmic reticulum-associated, interferon-inducible
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: MaP.VIP, monoclonal
• Application: FACS, ICC, IHC, IP, WB
• Citations: 21

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MaP.VIP, monoclonal
• species reactivity: human, rat, mouse
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_542388
• UniProt accession no.: Q8WXG1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RSAD2(91543)

Description

General description

Virus inhibitory protein endoplasmic reticulum-associated interferon-inducible (Viperin) belongs to the RSAD2 family and is highly inducible by both type I and type II interferons, but little or no induction is observed in moncytic cell lines. Studies suggest that viperin is involved in host antiviral response, as viperin induction is observed from viral infections. Research also reveals that viperin inhibits influenza replication by disrupting its release from the plasmic membrane through the altering of plasma membrane fluidity by affecting formation of lipid rafts. Lipid rafts are known detergent-resistant microdomian sites for influenza budding.

~42 kDa observed

Immunogen

Recombinant protein corresponding to human Viperin.

Application

Anti-Viperin Antibody, clone MaP.VIP detects level of Viperin & has been published & validated for use in WB, IH, FC, IP & IC.

Immunohistochemistry Analysis: A 1:500-1:1,000 dilution from a representative lot detected Viperin in human colon and rat liver tissues.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HepG2 cell lysate (Hinson, E., et al. (2008). JBC. 284(7):4705–4712).

Flow Cytometry Analysis: A representative lot was used by an independent laboratory in mouse liver, spleen, lung, and lymph node cells (Hinson, E., et al. (2010). J. Immunol. 184:5723-5731).

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in mouse spleen cells (Hinson, E., et al. (2010). J. Immunol. 184:5723-5731).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse macrophage stimulated with IFN gamma and LPS

Evaluated by Western Blot in mouse macrophage stimulated with IFN gamma and LPS.

Western Blot Analysis: 1 µg/mL of this antibody detected Viperin in 10 µg of mouse macrophage stimulated with IFN gamma and LPS.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable