1.08487

Urea

GR for analysis ACS,Reag. Ph Eur

• Synonym(s): Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• UNSPSC Code: 12352111
• NACRES: NA.21
• EC Index Number: 200-315-5
• MDL number: MFCD00008022
• Beilstein/REAXYS Number: 635724

Properties

• grade: ACS reagent
• Quality Level: 200
• agency: reag. Ph. Eur.
• vapor pressure: <0.1 hPa ( 20 °C)
• form: solid
• potency: 8471 mg/kg LD₅₀, oral (Rat), 8200 mg/kg LD₅₀, skin (Rat)
• technique(s): titration: suitable
• pH: 9 (20 °C, 100 g/L in H₂O)
• mp: 132-135 °C (lit.)
• density: 1.335 g/mL at 25 °C (lit.)
• bulk density: 720‑760 kg/m³
• storage temp.: 15-25°C
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Analysis Note

Assay (ex N): 99.0 - 100.5 %
Assay (ex N, calc. on dried substance): 99.0 - 101.5 %
Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %
Identity (IR-spectrum): passes test
Appearance of solution (10 %; Wasser; colour): colourless
Appearance of solution (10 %; Wasser; clarity): clear
In water insoluble matter: ≤ 0.003 %
Acidity, Alkalinity: ≤ 0.0005 meq/g
Melting point (DSC): 132 - 135 °C
NH₄ (Ammonium): ≤ 0.0500 %
Chloride (Cl): ≤ 0.0005 %
Sulfate (SO₄): ≤ 0.001 %
Heavy metals (as Pb): ≤ 0.0004 %
Biuret: ≤ 0.05 %
Sulfated ash (600 °C): ≤ 0.005 %
Cu (Copper): ≤ 0.0001 %
Fe (Iron): ≤ 0.0001 %
Pb (Lead): ≤ 0.0002 %
Loss on Drying (105°C): ≤ 1.0 %
Corresponds to ACS, Reag. Ph Eur

Other Notes

Replaces: UX0065-1

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable