Key Documents

View All Documentation

Lipopolysaccharides from Salmonella enterica serotype enteritidis

Name: Lipopolysaccharides from <I>Salmonella enterica</I> serotype enteritidis
Brand: SIGMA

purified by gel-filtration chromatography

Synonym(s):

LPS

Select a Size

Change View

Pack Size	SKU	Availability	Price

About This Item

UNSPSC Code:

12352201

NACRES:

NA.25

MDL number:

MFCD00131520

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

Salmonella enterica (Serotype enteritidis)

Quality Level

200

form

lyophilized powder

purified by

gel-filtration chromatography

impurities

<1% Protein

color

white to faint yellow

solubility

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

Description

General description

This product is extracted from Salmonella enteritidis and purified by gel filtration. The source strain is ATCC 13076. Lipopolysaccharide from Salmonella enteritidis is mutagenic in murine spleen cells and causes a plaque-forming response in those cells.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	L4774	L6011	L2262
Sigma-Aldrich L2012 Lipopolysaccharides from Salmonella enterica serotype enteritidis	Sigma-Aldrich L4774 Lipopolysaccharides from Salmonella enterica serotype enteritidis	Sigma-Aldrich L6011 Lipopolysaccharides from Salmonella enterica serotype enteritidis	Sigma-Aldrich L2262 Lipopolysaccharides from Salmonella enterica serotype typhimurium
biological source Salmonella enterica (Serotype enteritidis)	biological source Salmonella enterica (Serotype enteritidis)	biological source Salmonella enterica (Serotype enteritidis)	biological source Salmonella enterica (Serotype typhimurium)
solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow	solubility -	solubility water: 0.90-1.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow	solubility water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
color white to faint yellow	color -	color white to faint yellow	color white to faint yellow

Still not finding the right product?

Explore all of our products under Lipopolysaccharides from Salmonella enterica serotype enteritidis

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Toll-like receptor 4 signaling is required for induction of gluconeogenic gene expression by palmitate in human hepatic carcinoma cells.

Laman K Mamedova et al.

The Journal of nutritional biochemistry, 24(8), 1499-1507 (2013-03-08)

Saturated free fatty acids (FFA) can activate inflammatory cascades including the toll-like receptor 4 (TLR4) pathway. TLR4 is expressed by hepatocytes and may help link FFA to altered hepatic gluconeogenesis in type 2 diabetes mellitus. This study examined the role

Therapeutic inhibition of CXCR2 by Reparixin attenuates acute lung injury in mice.

A Zarbock et al.

British journal of pharmacology, 155(3), 357-364 (2008-07-01)

Acute lung injury (ALI) remains a major challenge in critical care medicine. Both neutrophils and chemokines have been proposed as key components in the development of ALI. The main chemokine receptor on neutrophils is CXCR2, which regulates neutrophil recruitment and

Peroxisome proliferator activated receptor gamma is not necessary for the development of LPS-induced tolerance in macrophages.

Basilia Zingarelli et al.

Immunology, 124(1), 51-57 (2007-11-22)

Peroxisome proliferator activated receptor-gamma (PPARgamma) has been reported to exert anti-inflammatory properties in endotoxic shock and sepsis. One phenomenon that alters the inflammatory response to endotoxin [lipopolysaccharide (LPS)] is endotoxin tolerance, which is caused by previous exposure to endotoxin. Here

View All Related Papers

Global Trade Item Number

SKU	GTIN
L2012-10MG	04061833272015
L2012-25MG	04061833289761

Questions

Reviews

No rating value