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F0504

Folate binding protein from bovine milk

lyophilized powder

Synonym(s):

Folate Protein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.61
MDL number:
MFCD00131098
Form:
lyophilized powder
Biological source:
bovine milk
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biological source

bovine milk

Quality Segment

200

form

lyophilized powder

usage

 mg sufficient for ~4,000 assays

composition

Protein, 20-40% modified Warburg-Christian

technique(s)

ligand binding assay: suitable, surface plasmon resonance (SPR): suitable

capacity

1.5-6.0 μg/mg, protein binding capacity (folic acid)(25 °C at pH 7.5.)

storage temp.

−20°C

Application

Folate binding protein can be used in radioligand binding assays of serum folate. The folate receptor has been used in studies as a therapeutic target in a variety of cancers.
Useful for radioligand binding assays of serum folate. May also bind N-methyltetrahydrofolic acid and other folate analogues.[1][2][3]
Folate binding protein from bovine milk has been used in folate binding studies using surface plasmon resonance and in the uptake studies in kidney proximal tubule cells.

Biochem/physiol Actions

Folate-binding protein (FBP) is present as a cell receptor and binds to folate in equimolar ratio. It is involved in the transport of folate and improves their bioavailability.

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing buffer salt as sodium citrate

Analysis Note

Purified over 1,000 fold. May contain a trace of β-mercaptoethanol.

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This Item
A7030C8654P1431
Folate binding protein from bovine milk lyophilized powder

Sigma-Aldrich

F0504

Folate binding protein from bovine milk

Bovine Serum Albumin heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%

Sigma-Aldrich

A7030

Bovine Serum Albumin

Casein sodium salt from bovine milk

Sigma-Aldrich

C8654

Casein sodium salt from bovine milk

Calmodulin from bovine testes BioUltra, ≥98% (SDS-PAGE), lyophilized powder, essentially salt free

Sigma-Aldrich

P1431

Calmodulin from bovine testes

technique(s)

ligand binding assay: suitable, surface plasmon resonance (SPR): suitable

technique(s)

ELISA: suitable, blood typing: suitable, ligand binding assay: suitable

technique(s)

electrophoresis: suitable, immunocytochemistry: suitable, ligand binding assay: suitable

technique(s)

ligand binding assay: suitable

biological source

bovine milk

biological source

bovine

biological source

bovine milk

biological source

bovine testis

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

capacity

1.5-6.0 μg/mg, protein binding capacity (folic acid)(25 °C at pH 7.5.)

capacity

-

capacity

-

capacity

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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