D3071

Dipeptidyl Peptidase IX human

recombinant, expressed in Sf9 cells

• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥250 units/μg protein
• Recombinant: expressed in Sf9 cells

Properties

• recombinant: expressed in Sf9 cells
• Quality Segment: 200
• form: solution
• specific activity: ≥250 units/μg protein
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Full length sequence with an N-terminal GST tag

Application

DPP9 is a protease of the DPP4 gene family that is used in cancer research to study the regulation of survival and proliferation pathways[1]. DPP9 is used to study cell-extracellular matrix interactions and tissue remodeling[2].

Biochem/physiol Actions

DPP IX is a member of the S9b family of dipeptidyl peptidases and cleaves the peptide hormones glucagon-like peptide-1, glucagon-like peptide-2, neuropeptide Y and peptide YY with marked kinetic differences compared with dipeptidyl peptidase IV. It has a role in regulating cell survival and proliferation through modulating molecular signaling cascades. Protein kinase B activation is significantly inhibited by human DPP9 overexpression in human hepatoma cells (HepG2 and Huh7) and human embryonic kidney cells (HEK293T)[1]. DPP9 is thought to influence cell-extracellular matrix interactions and may be involved in the regulation of tissue remodeling [2].

Physical form

Supplied as a solution in 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, and 16 mM glutathione

Other Notes

DPP IX is a member of the S9b family of dipeptidyl peptidases. and cleaves the peptide hormones glucagon-like peptide-1, glucagon-like peptide-2, neuropeptide Y and peptide YY with marked kinetic differences compared with dipeptidyl peptidase IV.

One unit will hydrolyze 1.0 picomole of H-Gly-Pro-AMC per minute at pH 7.5 at 25 deg °C

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1