35 年来，我们一直为 PCR、克隆、DNA 测序和基因表达应用提供定制和预设计寡核苷酸及 qPCR 探针。
我们提供用于寡核苷酸和 qPCR 探针设计的工具，包括OligoArchitect™引物和探针设计解决方案以及OligoEvaluator™寡核苷酸序列计算器，可提供PCR引物分析值，并帮助确定将DNA 寡核苷酸重悬和稀释至目标终浓度所需的体积。
推荐文章和实验方案
- 我们提供简单易用的OligoEvaluator™寡核苷酸分析工具，为PCR提供更准确的引物二聚体分析参数，并作为线上寡核苷酸序列计算器和Tm计算器。
- 了解寡核苷酸固相亚磷酰胺合成法的步骤。
- 退火是加热和冷却两条具有互补序列的单链寡核苷酸的过程。
技术文献
- 了解寡核苷酸固相亚磷酰胺合成法的步骤。
- 根据您的预期应用探索最适宜的寡核苷酸纯化方案。
- DNA 具有高度偶联的性质，可通过紫外光度计轻松定量。
- 硫代磷酸酯是一种广泛使用的主链变体，可有效产生用于反义敲低的寡核苷酸。
- The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular nuclease degradation.
- 查看采用常购定制qPCR探针进行的应用汇总。
- Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.
- 锁核酸 (LNA) 常见问题解答
- TAMRA™和DABYCL这两种常用淬灭剂限制了qPCR的最终灵敏度和灵活性。
- 通过操作处理和稳定性技术来减少变量并确保无故障实验。
- 了解寡核苷酸解链温度（Tm）的计算方法。
实验方案、操作步骤、安全和质量
- NHS-酯修饰与氨基标记的寡核苷酸缀合的实验方案
- 退火是加热和冷却两条具有互补序列的单链寡核苷酸的过程。
- 根据此DTT还原实验方案来还原硫醇修饰的寡核苷酸中的二硫键。
- Sigma制造流程的基础在于一套稳健的质量管理体系，其推动了向ISO 9001:2008认证的合规性。
- 我们致力于不断改善产品质量和生产效率，超越客户的期望。
视频
产品引文和出版文献
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Kudla G, Murray AW, Tollervey D, Plotkin JB. 2009. Coding-Sequence Determinants of Gene Expression in Escherichia coli. Science. 324(5924):255-258. https://doi.org/10.1126/science.1170160
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Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F. Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE. 4(12):e8291. https://doi.org/10.1371/journal.pone.0008291
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Omumi A, Beach DG, Baker M, Gabryelski W, Manderville RA. 2011. Postsynthetic Guanine Arylation of DNA by Suzuki?Miyaura Cross-Coupling. J. Am. Chem. Soc.. 133(1):42-50. https://doi.org/10.1021/ja106158b
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Pandey M, Syed S, Donmez I, Patel G, Ha T, Patel SS. 2009. Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature. 462(7275):940-943. https://doi.org/10.1038/nature08611
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Wang Z, Elbaz J, Remacle F, Levine RD, Willner I. 2010. All-DNA finite-state automata with finite memory. Proceedings of the National Academy of Sciences. 107(51):21996-22001. https://doi.org/10.1073/pnas.1015858107
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Yang L, Kemadjou JR, Zinsmeister C, Bauer M, Legradi J, Müller F, Pankratz M, Jäkel J, Strähle U. 2007. Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. Genome Biol. 8(10):R227. https://doi.org/10.1186/gb-2007-8-10-r227
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Zykovich A, Korf I, Segal DJ. 2009. Bind-n-Seq: high-throughput analysis of in vitro protein?DNA interactions using massively parallel sequencing. 37(22):e151-e151. https://doi.org/10.1093/nar/gkp802
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Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T. 2011. Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2?-O-methyl RNA probes in living cells. 39(4):e20-e20. https://doi.org/10.1093/nar/gkq1196
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Josefsen MH, Löfström C, Sommer HM, Hoorfar J. 2009. Diagnostic PCR: Comparative sensitivity of four probe chemistries. Molecular and Cellular Probes. 23(3-4):201-203. https://doi.org/10.1016/j.mcp.2009.02.003
2.
Saito Y, Kitamura H, Hijikata A, Tomizawa-Murasawa M, Tanaka S, Takagi S, Uchida N, Suzuki N, Sone A, Najima Y, et al. 2010. Identification of Therapeutic Targets for Quiescent, Chemotherapy-Resistant Human Leukemia Stem Cells. Science Translational Medicine. 2(17):17ra9-17ra9. https://doi.org/10.1126/scitranslmed.3000349
1.
Schutkowski A, König B, Kluge H, Hirche F, Henze A, Schwerdtle T, Lorkowski S, Dawczynski C, Gabel A, Große I, et al. 2019. Metabolic footprint and intestinal microbial changes in response to dietary proteins in a pig model. The Journal of Nutritional Biochemistry. 67149-160. https://doi.org/10.1016/j.jnutbio.2019.02.004
2.
Schutkowski A, Max D, Bönn M, Brandsch C, Grundmann SM, Hirche F, Staege MS, Stangl GI. 2018. Vitamin D Does Not Play a Functional Role in Adipose Tissue Development in Rodent Models. Mol. Nutr. Food Res.. 62(4):1700726. https://doi.org/10.1002/mnfr.201700726
3.
Kühne H, Hause G, Grundmann SM, Schutkowski A, Brandsch C, Stangl GI. 2016. Vitamin D receptor knockout mice exhibit elongated intestinal microvilli and increased ezrin expression. Nutrition Research. 36(2):184-192. https://doi.org/10.1016/j.nutres.2015.10.005
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Schutkowski A, Wege N, Stangl GI, König B. Tissue-Specific Expression of Monocarboxylate Transporters during Fasting in Mice. PLoS ONE. 9(11):e112118. https://doi.org/10.1371/journal.pone.0112118
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Kühne H, Schutkowski A, Weinholz S, Cordes C, Schierhorn A, Schulz K, König B, Stangl GI. 2014. Vitamin D receptor regulates intestinal proteins involved in cell proliferation, migration and stress response. Lipids Health Dis. 13(1):51. https://doi.org/10.1186/1476-511x-13-51
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Hudnall SD, Chen T, Allison P, Tyring SK, Heath A. 2008. Herpesvirus prevalence and viral load in healthy blood donors by quantitative real-time polymerase chain reaction. Transfusion. 48(6):1180-1187. https://doi.org/10.1111/j.1537-2995.2008.01685.x
*使用qPCR引物和探针检测血液样本中的病毒； 一位定制产品科学家合作撰写了这篇论文。
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