Hemacolor reagents are approved for In Vitro Diagnostic Use for human blood. Although stains used for human blood will often work with rodents and other mammals, no testing has been performed to document how well the Hemacolor reagents work with mouse blood or bone marrow. Bone marrow slides typically take longer to stain than peripheral blood smears, so separate procedures would need to be developed for fresh peripheral blood or bone marrow specimens.
1.11955
Hemacolor® Rapid staining of blood smear
Solution 1: fixing solution
Synonym(s):
Methanol, MeOH, Hydroxymethane, Methyl alcohol, Carbinol, Methyl alcohol
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About This Item
Linear Formula:
CH3OH
CAS Number:
Molecular Weight:
32.04
UNSPSC Code:
41116121
NACRES:
NA.21
EC Index Number:
200-659-6
MDL number:
Beilstein/REAXYS Number:
1098229
Pricing and availability is not currently available.
vapor density
1.11 (vs air)
Quality Segment
vapor pressure
128 hPa ( 20 °C), 410 mmHg ( 50 °C), 97.68 mmHg ( 20 °C)
form
liquid
autoignition temp.
725 °F
potency
5628 mg/kg LD50, oral (Rat), 17100 mg/kg LD50, skin (Rabbit)
expl. lim.
36 %
IVD
for in vitro diagnostic use
technique(s)
microbe id | staining: suitable
refractive index
n20/D 1.329 (lit.)
kinematic viscosity
0.54-0.59 cSt(20 °C)
bp
64.7 °C (lit.)
mp
−98 °C (lit.)
transition temp
flash point 9.7 °C
density
0.791 g/mL at 25 °C (lit.)
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
SMILES string
CO
InChI
1S/CH4O/c1-2/h2H,1H3
InChI key
OKKJLVBELUTLKV-UHFFFAOYSA-N
General description
Hemacolor® Rapid staining of blood smear - Solution 1: fixing solution, is a ready-to-use solution used for human-medical cell diagnosis and serves the fixing of native blood and bone-marrow smears of human origin in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow. The staining with Hemacolor® yields a staining result that corresponds to the Pappenheim stain, with predominantly magenta stained nuclei. This is based on the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. By using pH 7.2 buffered solutions with the Hemacolor® Rapid staining of blood smear solutions, a high stability of the stain and clean, precipitation-free staining results can be guaranteed.
The package is sufficient for 1250 - 5000 applications. The buffer tablets according to Weise pH 7.2 (Product number 1.09468) are also available in larger pack sizes, thus, they can be useful for higher demands. The three solutions needed for staining are all available individually as:
Hemacolor® Rapid staining of blood smear solution 1, fixing solution (Product number 1.11955)
Hemacolor® Rapid staining of blood smear solution 2, colour reagent red (Product number 1.11956)
Hemacolor® Rapid staining of blood smear solution 3, colour reagent blue (Product number 1.11957)
or they can be purchased as staining sets:
Hemacolor® Rapid staining of blood smear (Product number 1.11674) 3 x 100 ml
Hemacolor® Rapid staining of blood smear (Product number 1.11661) 3 x 500 ml
For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
The package is sufficient for 1250 - 5000 applications. The buffer tablets according to Weise pH 7.2 (Product number 1.09468) are also available in larger pack sizes, thus, they can be useful for higher demands. The three solutions needed for staining are all available individually as:
Hemacolor® Rapid staining of blood smear solution 1, fixing solution (Product number 1.11955)
Hemacolor® Rapid staining of blood smear solution 2, colour reagent red (Product number 1.11956)
Hemacolor® Rapid staining of blood smear solution 3, colour reagent blue (Product number 1.11957)
or they can be purchased as staining sets:
Hemacolor® Rapid staining of blood smear (Product number 1.11674) 3 x 100 ml
Hemacolor® Rapid staining of blood smear (Product number 1.11661) 3 x 500 ml
For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.
Legal Information
HEMACOLOR is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - | technique(s) HPLC: suitable |
| application(s) clinical testing | application(s) environmental | application(s) environmental | application(s) food and beverages |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 15-25°C | storage temp. 2-30°C | storage temp. 2-30°C | storage temp. 2-30°C |
| IVD for in vitro diagnostic use | IVD - | IVD - | IVD - |
| Quality Level 400 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Storage Class
3 - Flammable liquids
wgk
WGK 2
flash_point_f
49.5 °F - closed cup
flash_point_c
9.7 °C - closed cup
target_organs
Eyes,Central nervous system
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
67-56-1
CAS No.
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B48207-250ML | 04061837016103 |
| B48207-1L | 04061837016097 |
| 1119552500 | 04022536137629 |
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Can this kit be used on mouse blood and bone marrow?
1 answer-
Helpful?
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Can this kit also be used to produce blood smears where the blood was previously stored in EDTA tubes?
1 answer-
Yes, the Hemacolor reagents are suitable for use with samples anticoagulated with EDTA. If the sample is not anticoagulated, the smears must be made immediately upon the blood being drawn. These reagents are not suitable for use with samples anticoagulated with heparin or sodium citrate. Smears should be prepared from fresh samples when prepared from anticoagulated blood with EDTA.
Helpful?
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