P4762

Papain from papaya latex

lyophilized powder, ≥10 units/mg protein

• Synonim(y): Papainase
• Numer CAS: 9001-73-4
• Numer EC enzymu: 3.4.22.2 (BRENDA, IUBMB)
• Numer WE: 232-627-2
• Numer MDL: MFCD00131791
• Kod UNSPSC: 12352204
• eCl@ss: 42030311
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: papaya (latex)
• Poziom jakości: 300
• Postać: lyophilized powder
• aktywność właściwa: ≥10 units/mg protein
• masa cząsteczkowa: 23.4 kDa
• metody: cell culture | mammalian: suitable; single cell analysis: suitable
• Zastosowanie: diagnostic assay manufacturing
• temp. przechowywania: −20°C

Opis

Opis ogólny

Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.

Zastosowanie

Papain from papaya latex has been used:

• to digest carotid artery smooth muscle cells
• in dissecting solutions
• to produce Fab fragments of antibodies
• for cell dissociation since it has been shown to be more effective and less damaging with certain tissues
• in the enzyme cocktail for dissociating human fetal retinal tissue
• to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation
• as a supplement in neurobasal medium to produce retinal cell suspensions

Suitable for use in preparation of single cell suspension for sequencing.

Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Działania biochem./fizjol.

Papain with wide specificity, cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases. Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues. The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants. Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.

• The pH optimum is 6.0-7.0
• Papain hydrolyzes esters and amides

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Opakowanie

Package size based on protein content

Definicja jednostki

One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

Postać fizyczna

Lyophilized powder containing sodium acetate

Uwaga dotycząca przygotowania

2× Crystallized

Inne uwagi

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H334,H335
• Zwroty wskazujące środki ostrożności: P302 + P352 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Faceshields, Gloves