10108014001
Roche
Papain
from Carica papaya
Synonim(y):
cystein protease, papain
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About This Item
Polecane produkty
pochodzenie biologiczne
papaya
Poziom jakości
Postać
suspension
aktywność właściwa
30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)
masa cząsteczkowa
23 kDa
opakowanie
pkg of 10 mL (100 mg)
producent / nazwa handlowa
Roche
stężenie
10-100 mg/mL
optymalne pH
6.0-7.0
Warunki transportu
wet ice
Powiązane kategorie
Opis ogólny
Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.
Zastosowanie
Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.
Działania biochem./fizjol.
Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.
Cechy i korzyści
Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals
Postać fizyczna
Suspension, crystalline, nonsterile
Uwaga dotycząca przygotowania
Working concentration: 0.05 to 0.5 mg/ml
Przechowywanie i stabilność
Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
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