L9641

Lipopolysaccharides (rough strains) from Escherichia coli EH100 (Ra mutant)

• Synonim(y): LPS
• Numer WE: 297-473-0
• Numer MDL: MFCD00164401
• Kod UNSPSC: 12352201
• NACRES: NA.25

Właściwości

• pochodzenie biologiczne: Escherichia coli (EH100 Ra mutant)
• Poziom jakości: 200
• Postać: lyophilized powder
• zanieczyszczenia: <3% Protein (Lowry)
• kolor: white to yellow
• rozpuszczalność: water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

This product is phenol:chloroform:petroleum ether extracted from E. coli EH 100 (Ra mutant). The source strain is from a private collection.

Działania biochem./fizjol.

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Uwaga dotycząca przygotowania

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Inne uwagi

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H300
• Zwroty wskazujące środki ostrożności: P264 - P270 - P301 + P310 - P405 - P501
• Klasyfikacja zagrożeń: Acute Tox. 2 Oral
• Kod klasy składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)