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L5543

Lipopolysaccharides from Escherichia coli O26:B6

Ready Made solution, 1 mg/mL, 0.2 μm filtered

Synonim(y):

LPS

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Do Państwa/SKUDostępnośćCena netto
2 mL
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1390,00 zł
1042,50 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:

1042,50 zł

Cena katalogowa1390,00 złZaoszczędź 25%

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

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biological source

Escherichia coli (O26:B6)

Quality Segment

sterility

0.2 μm filtered

form

aqueous solution

concentration

1 mg/mL

color

colorless to faint yellow

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

General description

This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O26:B6. This LPS serotype has been used in studies of septic shock.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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Ta pozycja
L5418L3012L3755
description

Ready Made solution, 1 mg/mL, 0.2 μm filtered

description

Ready Made solution, 1 mg/mL

description

purified by gel-filtration chromatography

description

purified by trichloroacetic acid extraction

biological source

Escherichia coli (O26:B6)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O111:B4)

biological source

Escherichia coli (O26:B6)

solubility

water: soluble

solubility

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

solubility

water: soluble

solubility

water: soluble

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

aqueous solution

form

aqueous solution

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

colorless to faint yellow

color

white to faint yellow

color

white to faint yellow

color

white to yellow


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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 1

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Questions

  1. What buffer is L5543, LPS in ?

    1 answer
    1. The LPS is in an aqueous solution containing 0.5% triethylamine and pH adjusted to 6.5 using hydrochloric acid.

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