L2637

Lipopolysaccharides from Escherichia coli O55:B5

purified by gel-filtration chromatography

• Synonim(y): LPS
• Numer WE: 297-473-0
• Numer MDL: MFCD00164401
• Kod UNSPSC: 12352201
• NACRES: NA.25

Właściwości

• pochodzenie biologiczne: Escherichia coli (O55:B5)
• Poziom jakości: 200
• Postać: lyophilized powder
• oczyszczone przez: gel-filtration chromatography
• zanieczyszczenia: <3% Protein
• kolor: white to faint gray
• rozpuszczalność: water: soluble
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

This product is extracted from E. coli serotype O55:B5 and purified by gel filtration. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/ml and to stimulate equine peritoneal macrophages at 1-100 ng/ml.

Zastosowanie

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Działania biochem./fizjol.

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Uwaga dotycząca przygotowania

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Inne uwagi

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H300
• Zwroty wskazujące środki ostrożności: P264 - P270 - P301 + P310 - P405 - P501
• Klasyfikacja zagrożeń: Acute Tox. 2 Oral
• Kod klasy składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)