L2762

Lipopolysaccharides from Escherichia coli O26:B6

purified by gel-filtration chromatography

• Synonim(y): LPS
• Numer CAS: 93572-42-0
• UNSPSC Code: 12352201
• EC Number: 297-473-0
• NACRES: NA.25
• MDL number: MFCD00164401

Właściwości

• biological source: Escherichia coli (O26:B6)
• Quality Segment: 200
• form: lyophilized powder
• purified by: gel-filtration chromatography
• impurities: <5% Protein
• color: white to faint yellow
• solubility: water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow
• shipped in: ambient
• storage temp.: 2-8°C

Opis

General description

This product is extracted from E. coli serotype O26:B6 and purified by gel filtration. The source strain is ATCC 12795. LPS from serotype 026:B6 is the only LPS from E. coli which exhibits short chain-length behavior on SDS-PAGE. The short chain-length of this LPS is closer to that of the mutant rough strain LPS. This LPS serotype has been used at 1 μg/ml to stimulate neutrophils.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H300
• Ostrzeżenia: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Klasa składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable