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83264

Sigma-Aldrich

HEPES solution

1 M in H₂O, liquid

Synonim(y):

HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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100 ML
485,00 zł
500 ML
1730,00 zł

485,00 zł

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100 ML
485,00 zł
500 ML
1730,00 zł

About This Item

Numer CAS:
7365-45-9
Numer MDL:
MFCD00006158
Kod UNSPSC:
12161700
eCl@ss:
32129211
Identyfikator substancji w PubChem:
57652951
NACRES:
NA.25

485,00 zł

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Nazwa produktu

HEPES buffer solution, 1 M in H2O

Formularz

liquid

stężenie

1 M in H2O
238 g/L HEPES in H2O

zanieczyszczenia

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

5.5±0.5

przydatny zakres pH

6.8-8.2

gęstość

1.07 g/mL at 20 °C

Zastosowanie

diagnostic assay manufacturing

ciąg SMILES

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

Klucz InChI

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Opis ogólny

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Zastosowanie

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum[3]
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI[4][5]
  • As a component of dissection buffer for culturing rat cortical neurons[6]
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses[7]
  • As a component of Danieu′s aquarium water used in agarose gel preparation[8]
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
BCCM1721
BCCM6251
BCCM3364
BCCL7705
BCCK7120

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Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

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Sigma-Aldrich

H7523

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EmbryoMax® 1M HEPES Buffer Solution, Liquid, 1M The EmbryoMax 1M HEPES Buffer Solution, Liquid, 1M is available in a 100 mL format and may be used for routine mouse embryonic stem cell culture applications.

Sigma-Aldrich

TMS-003

EmbryoMax® 1M HEPES Buffer Solution, Liquid, 1M

HEPES sodium salt ≥99.0% (titration)

Sigma-Aldrich

H7006

HEPES sodium salt

HEPES ULTROL® Grade, ≥99% dry basis (titration), 1M zwitterionic N-substituted aminosulfonic acid buffer

Millipore

375368

HEPES

HEPES anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD002

HEPES

HEPES potassium salt ≥99.5% (titration)

Sigma-Aldrich

H0527

HEPES potassium salt

Novel membrane-based electrochemical sensor for real-time bio-applications.
Alatraktchi FA, et al.
Sensors, 14(11), 22128-22139 (2014)
Cultivation of Salmonella enterica serovar Typhimurium in a norepinephrine-containing medium alters in vivo tissue prevalence in swine.
Toscano MJ, et al.
Journal of experimental animal science, 43 , 329-338 (2007)
Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures.
Zhao X, et al.
Bio-protocol, 6(8) (2016)
"Hypoxia-induced metastasis model in embryonic zebrafish.
Rouhi P, et al.
Nature Protocols, 5(12), 1911-1911 (2010)
Group 2 innate lymphoid cells express functional NKp30 receptor inducing type 2 cytokine production.
Salimi M, et al.
Journal of Immunology, 196(1), 45-54 (2016)
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Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can the pH be raised from 5.5 to 8.3?

    1 answer

    1. To increase the pH from 5.5 to 8.3, you can use 0.1 M NaOH.

      Helpful?

  4. 해당 HEPES buffer solution 에 HEPES 분자 외에 다른 salt 들 (ex. Na, K 등) 은 없는 상태인가요? 단순히 물에 HEPES 분자가 풀려있는 상황인지 궁금합니다.

    1 answer

    1. This product is comprised only of HEPES in water. There are no other salts or additives present in the material.

      Helpful?

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