RDD002

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri^™

• Synonim(y): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Wzór empiryczny (zapis Hilla): C₈H₁₈N₂O₄S
• Numer CAS: 7365-45-9
• Masa cząsteczkowa: 238.30
• Beilstein: 883043
• Numer WE: 230-907-9
• Numer MDL: MFCD00006158
• Kod UNSPSC: 12161700
• Identyfikator substancji w PubChem: 329824215
• NACRES: NA.25

Właściwości

• Nazwa produktu: HEPES, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%
• klasa czystości: anhydrous
• Poziom jakości: 100
• linia produktu: Redi-Dri^™
• Próba: ≥99.5%
• Formularz: crystalline powder
• jakość: free-flowing
• pH: 5.0-6.5 (25 °C, 238 g/L)
• przydatny zakres pH: 6.8-8.2
• pKa (25°C): 7.5
• rozpuszczalność: water: 500 mg/mL, clear, colorless
• ciąg SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Klucz InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Opis

Opis ogólny

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Zastosowanie

HEPES has been used:

• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions[3]
• As a component in intracellular solution for the preparation of brain-slice recording[4]
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification[5]
• To maintain the pH at 7 in thermal denaturation assay of purified protein[5]
• In isocratic elution of protein using gel filtration[5]
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy[5]

Informacje prawne

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable