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H3537

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Synonim(y):

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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Do Państwa/SKUDostępnośćCena netto
100 mL
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
697,00 zł
500 mL
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2020,00 zł
1 L
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3600,00 zł
3060,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
MDL number:

697,00 zł


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Nazwa produktu

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

grade

BioPerformance Certified

Quality Segment

sterility

0.2 μm filtered

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

cation traces

Fe: <5 ppm, heavy metals (as Pb): <5 ppm

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

RNAse HEPES has been used:
  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
  • To recover purified ribonucleotide[1]
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets[2]
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Ta pozycja
H0887H088783264
description

BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

description

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

description

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

description

1 M in H2O

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

grade

BioPerformance Certified

grade

-

grade

-

grade

-

concentration

1 M

concentration

1 M

concentration

1 M

concentration

1 M in H2O, 238 g/L HEPES in H2O

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

100

application(s)

diagnostic assay manufacturing

application(s)

advanced drug delivery
cell analysis
life science and biopharma
sample preparation

application(s)

advanced drug delivery
cell analysis
life science and biopharma
sample preparation

application(s)

diagnostic assay manufacturing

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

grade

BioPerformance Certified

grade

-

grade

-

grade

-

concentration

1 M

concentration

1 M

concentration

1 M

concentration

1 M in H2O, 238 g/L HEPES in H2O

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

100

application(s)

diagnostic assay manufacturing

application(s)

advanced drug delivery
cell analysis
life science and biopharma
sample preparation

application(s)

advanced drug delivery
cell analysis
life science and biopharma
sample preparation

application(s)

diagnostic assay manufacturing

form

liquid

form

liquid

form

liquid

form

liquid


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Klasa składowania

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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