H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

• Synonim(y): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Wzór empiryczny (zapis Hilla): C₈H₁₈N₂O₄S
• Numer CAS: 7365-45-9
• Masa cząsteczkowa: 238.30
• Beilstein: 883043
• Numer WE: 230-907-9
• Numer MDL: MFCD00006158
• Kod UNSPSC: 12161700
• Identyfikator substancji w PubChem: 24895796
• NACRES: NA.25

Właściwości

• linia produktu: BioXtra
• Próba: ≥99.5% (titration)
• Postać: powder
• zanieczyszczenia: Insoluble matter, passes filter test
• pozostałość po prażeniu (900°C): ≤0.1% (as SO₄)
• strata: ≤1.0% loss on drying, 110°C
• pH: 5.0-6.5 (1 M in H₂O)
• przydatny zakres pH: 6.8-8.2
• pKa (25°C): 7.5
• rozpuszczalność: H₂O: 1 M, clear, colorless
• ślady anionów: chloride (Cl^-): ≤0.05%
• ślady kationów: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• absorpcja: ≤0.035 at 280 in H₂O at 1 M; ≤0.045 at 260 in H₂O at 1 M
• Zastosowanie: diagnostic assay manufacturing
• ciąg SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Klucz InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Opis

Opis ogólny

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Zastosowanie

HEPES has been used:

• As a component of homogenisation buffer used in protein sample preparation
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Inne uwagi

Easily compare specifications for HEPES products with the HEPES specification table.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)