10745731001

β-Galactosidase

EIA grade

• Synonim(y): β galactosidase, β-galactosidase
• Numer EC enzymu: 3.2.1.23 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: Escherichia coli
• Poziom jakości: 100
• Próba: (single peak, HPLC)
• Postać: lyophilized
• aktywność właściwa: 750-950 U/mg
• masa cząsteczkowa: 540.000 kDa
• opakowanie: pkg of 25 mg (approx. 100 mg lyophilizate)
• producent / nazwa handlowa: Roche
• metody: enzyme immunoassay: suitable
• kolor: white
• pH: 7.3-7.7
• rozpuszczalność: water: 20 mg/mL
• λmaks.: 405
• przydatność: suitable for immunoassay
• numer dostępu NCBI: WP_050940144
• numer dostępu UniProt: P00722; ; ; ; P00722
• Zastosowanie: life science and biopharma
• Warunki transportu: dry ice
• temp. przechowywania: −20°C (−15°C to −25°C)
• informacje o genach: Escherichia coli ... lacZ(945006)

Opis

Opis ogólny

β-Galactosidase, EIA grade, is a lyophilizate from E. coli overproducer, consisting of enzyme protein, phosphate buffer, and sucrose. Substances which could interfere with the derivatization of NH₂ or SH groups (e.g., 2-mercaptoethanol, ammonium salts, primary amines etc.) have been removed.

The GLB1 (β-Galactosidase) gene is mapped to human chromosome 3p22.3. The encoded protein belongs to glycosyl hydrolase 35 family and is localized to lysosomes.

Zastosowanie

β-Galactosidase has been used as a standard to determine the absolute quantitation of LacZ protein.

β-Galactosidase is used for labeling enzyme immunoassay techniques. β-Galactosidase can be coupled to other proteins via its SH-groups. The reconstituted solution can be used directly for conjugation without prior dialysis or gel permeation chromatography.

Działania biochem./fizjol.

β-Galactosidase catalyzes the hydrolysis of terminal β-galactose residue of ganglioside substrates, such as glycoproteins, sphingolipids, and keratan sulfate and other glycoconjugates. This enzyme is associated with the mechanism of cell senescence and carcinogenesis. Mutations in the gene result in gangliosidosis, an autosomal recessive disorder, characterized with defective lysosomal storage due to accumulation of substrates. β-Galactosidase deficiency also causes Morquio B syndrome indicating skeletal abnormalities, short stature and increased excretion of keratan sulfate in urine.

Jakość

Purity: single peak (HPLC)

Sekwencja

Free Thiol Groups
The non-denatured, enzymatically-active preparation contains > 12 SH groups per molecule which are not involved in disulfide bridges and are freely accessible to coupling reagents in aqueous media (as assayed with Ellman′s reagent at +37 °C, acc. to Habeeb, 1972).

Absence of Enzyme Aggregates
The preparation contains < 3% dimers (HPLC, area %) and essentially no higher oligomers.

Postać fizyczna

Lyophilizate, stabilized with phosphate buffer and sucrose. Note: Contains at least 12 free SH-groups/enzyme molecule; 5 mg approximately 20 mg lyophilizate; 25 mg approximately 100 mg lyophilizate.

Przechowywanie i stabilność

Store at -15–-25 °C. (sealed under nitrogen)

Komentarz do analizy

Specific activity: approximately 750 - 950 U/mg enzyme protein ≅ approximately 150 - 250 U/mg lyophilizate (+37°C, 2-nitrophenyl-β-D-galactoside); approximately 250-400 U/mg enzyme protein ≅ approximately 60-100 U/mg lyophilizate (+37°C, 4-nitrophenyl-β-D-galactoside).

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash