10109045001

Kinaza pirogronianowa (PK)

from rabbit muscle

• Synonim(y): PK
• Numer EC enzymu: 2.7.1.40 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: rabbit muscle
• Poziom jakości: 100
• Formularz: solution
• aktywność właściwa: ~200 units/mg protein (At 25 °C with PEP as the substrate.)
• opakowanie: pkg of 1 mL (10 mg)
• producent / nazwa handlowa: Roche
• optymalne pH: 7.0-7.5
• Warunki transportu: dry ice
• temp. przechowywania: −20°C (−15°C to −25°C)

Opis

Opis ogólny

Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn²⁺ and phosphoenolpyruvate.[1]

Specyficzność

Specific activity: Approximately 200 U/mg at +25°C with PEP as the substrate.

Zastosowanie

Pyruvate Kinase (PK) has been used as a sample in ATPase Assays.[2][3]

Pyruvate Kinase (PK) has been used in the kinase assay.[4]

Działania biochem./fizjol.

Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. The enzyme also exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.[5]

Jakość

Contaminants: <0.001% GK, <0.002% HK, “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each.

Postać fizyczna

Solution in 50% glycerol (v/v), pH approximately 6

Uwaga dotycząca przygotowania

Activator: PK requires Mg²⁺ (or Mn²⁺, Co²⁺) and K⁺ (or NH⁴⁺, Rb⁺) for full activity.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): No data available
• Temperatura zapłonu (°C): No data available