おすすめの製品
グレード
Molecular Biology
for molecular biology
フォーム
buffered aqueous glycerol solution
分子量
28 kDa
UniProtアクセッション番号
保管温度
−20°C
遺伝子情報
Escherichia coli K12 ... xthA(946254)
詳細
A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA, therefore reaction conditions must be optimized for specific applications.
アプリケーション
Suitable for:
- Production of strand specific probes
- Preparation of single stranded templates for Sanger dideoxy sequencing
- Site directed mutagenesis
構成
Exonuclease III is supplied as a solution in 5 mM potassium phosphate (pH 6.5), 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol.
単位の定義
1 unitは、 37°C、30分間に、1 nmolの酸可溶性ヌクレオチドを超音波処理した仔ウシ胸腺DNAから遊離させる酵素量です。
関連製品
製品番号
詳細
価格
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
E1131-5KU:4548173297804
E1131-2.5KU:
E1131-2.5KU-KC:
E1131-20KU:
E1131-BULK:
E1131-VAR:
Ying Chen et al.
Chemical communications (Cambridge, England), 47(48), 12798-12800 (2011-11-15)
The presence of exonuclease III leads to direct recycling and reuse of the target DNA, which in turn results in substantial signal amplification for highly sensitive, label-free impedimetric detection of specific DNA sequences.
Peng Hu et al.
Biosensors & bioelectronics, 34(1), 83-87 (2012-03-03)
We report here a graphene oxide (GO)-based fluorescent aptasensor for adenosine detection by employing exonuclease III (Exo III) as a signal amplifying element. In the absence of adenosine, the adenosine aptamers hybridized with the complementary DNA (cDNA), and the Exo
Sai Bi et al.
Chemical communications (Cambridge, England), 48(7), 1018-1020 (2011-12-07)
The network consisting of three kinds of unlabeled stem-loop DNA molecular beacons (MBs) is activated by target DNA in the presence of exonuclease-III (Exo-III), achieving the concept of exonuclease-assisted cascaded recycling amplification (Exo-CRA) for DNA detection with a wide dynamic
L H Guo et al.
Nucleic acids research, 10(6), 2065-2084 (1982-03-25)
We describe improve enzymatic methods for sequencing method for sequencing DNA. They are based on partial digestion of duplex DNA with exonuclease III to produce DNA molecules with 3' ends shortened to varying lengths, followed by repair synthesis to extend
Xiaofeng Wei et al.
Chemical communications (Cambridge, England), 48(49), 6184-6186 (2012-05-17)
Based on the specific folic acid-folate receptor (FA-FR) interaction, macromolecular FR can bind with FA-linked DNA-small molecule chimeras, which can prevent enzymolysis by exonuclease III (Exo III), enabling a novel fluorescence biosensor for FR to be developed using quinaldine red
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）