D9380

DNAポリメラーゼI from Escherichia coli lysogenic for NM 964

buffered aqueous glycerol solution

• 別名: コーンバーグポリメラーゼ
• CAS番号: 9012-90-2
• UNSPSC Code: 12352204
• NACRES: NA.53
• EC Number: 2.7.7.7 (BRENDA, IUBMB)
• MDL number: MFCD00130924
• Concentration: 5,000-15,000 units/mL

特性

• grade: Molecular Biology
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• mol wt: 109 kDa
• concentration: 5,000-15,000 units/mL
• UniProt accession no.: P00582
• foreign activity: Endonuclease, none detected
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: Escherichia coli K12 ... polA(948356)

詳細

General description

DNA polymerase I (holoenzyme) has 5′→3′ and 3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced.

Application

Suitable for:

• Highly specific DNA probes by nick translation
• In vitro synthesis of complementary cDNA strand
• In vitro synthesis of DNA
• Produce blunt ends from 5′ and 3′ overhangs

DNA Polymerase I from Escherichia coli has been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity.

Other Notes

1 unitは、37°C、30分間に、10 nmolのデオキシリボヌクレオシド三リン酸を酸不溶性物質に変換する酵素量です。

DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate buffer (pH 6.5), and 1 mM dithiothreitol.

安全性情報

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• 保管分類: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)