おすすめの製品
リコンビナント
expressed in E. coli
グレード
for molecular biology
形状
buffered aqueous solution
分子量
26 kDa
濃度
>0.1 mg/mL
>1 unit/mL
UniProtアクセッション番号
輸送温度
wet ice
保管温度
2-8°C
遺伝子情報
human ... GSTM1(2944)
詳細
Glutathione S-transferase (GST) catalyzes the addition of the glutathione thiol group to a suitable electrophilic species. Enzymatic activities are based on the conjugation of reduced glutathione in the presence of a second substrate.
特異性
Glutathione S-transferase (GST) catalyzes the addition of the glutathione thiol group to a suitable electrophilic species. Enzymatic activities are based on the conjugation of reduced glutathione in the presence of a
second substrate.
In ELISA, 0.5 μg of recombinant glutathione S-transferase is the minimum detectable level of enzyme with an anti-glutatione S-transferase, alkaline phosphatase conjugate.
In immunoblot, 50 ng of recombinant GST is the minimum detectable level of enzyme with an anti-glutathione S-transferase, alkaline phosphatase conjugate.
second substrate.
In ELISA, 0.5 μg of recombinant glutathione S-transferase is the minimum detectable level of enzyme with an anti-glutatione S-transferase, alkaline phosphatase conjugate.
In immunoblot, 50 ng of recombinant GST is the minimum detectable level of enzyme with an anti-glutathione S-transferase, alkaline phosphatase conjugate.
アプリケーション
Glutathione S-Transferase (GST) from E. coli has been used in the standard curve preparation for the quantification of GST-V5H6 produced by enzyme-linked immunosorbent assay (ELISA).
Suitable for use in ELISA and Western blot applications.
生物化学的/生理学的作用
Glutathione S-transferases (GST) catalyzes the conjugation of reduced glutathione with several substrates, which leads to detoxification. It also serves as transport proteins.
警告
Due to the sodium azide content, consult the SDS for information regarding hazards and safe handling practices.
単位の定義
One unit will conjugate 1.0 micromole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per minute at pH 6.5 at 25 deg C.
物理的形状
PBS溶液 (0.02%アジ化ナトリウム含有)
関連製品
製品番号
詳細
価格
保管分類コード
12 - Non Combustible Liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
G5663-.1MG-KC:
G5663-BULK:
G5663-.1MG:
G5663-1VL-LBL:
G5663-VAR:
G5663-1VL:
G5663-5MG:
G5663-1VL-PW:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
この製品を見ている人はこちらもチェック
Living recombinant Saccharomyces cerevisiae secreting proteins or peptides as a new drug delivery system in the gut
Journal of Biotechnology, 110(1), 37-49 (2004)
Methods in molecular biology (Clifton, N.J.), 328, 1-14 (2006-06-21)
Protein microarrays are a miniaturized format for displaying in close spatial density hundreds or thousands of purified proteins that provide a powerful platform for the high-throughput assay of protein function. The traditional method of producing them requires the high-throughput production
Clinical pathology in non-clinical toxicology testing
Haschek and Rousseaux's Handbook of Toxicologic Pathology, 565-594 (2013)
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
Gene, 67(1), 31-40 (1988)
Methods in enzymology, 500, 151-163 (2011-09-29)
Systematic study of proteins requires the availability of thousands of proteins in functional format. However, traditional recombinant protein expression and purification methods have many drawbacks for such study at the proteome level. We have developed an innovative in situ protein
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)