C0150

カテプシンB ヒト胎盤由来

lyophilized powder, ≥5 units/mg protein

• 別名: カテプシンB₁
• CAS番号: 9047-22-7
• Enzyme Commission number: 3.4.22.1 (BRENDA, IUBMB)
• MDL番号: MFCD00130748
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• フォーム: lyophilized powder
• 品質水準: 200
• 比活性: ≥5 units/mg protein
• 分子量: 24.5 kDa
• 組成: Protein, ~50% Lowry
• 溶解性: H₂O: soluble 1.0 mg/mL, clear
• UniProtアクセッション番号: P07858
• 保管温度: −20°C
• 遺伝子情報: human ... CTSB(1508)

詳細

詳細

Cathepsin B is encoded by the gene CTSB and is located in the human chromosome at 8p23.1.[1] Cathepsin B is a lysosomal proteolytic enzyme of cysteine protease family. It is present in all mammalian cells. It is essential for the intracellular protein turnover.[2]

アプリケーション

Cathepsin B from human placenta has been used in the in vitro digestion of modified myelin oligodendrocyte glycoprotein (MOG).[3] It has also been used in determining the effect of cysteine protease inhibitors.[2]

Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease [4]. Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

生物化学的／生理学的作用

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments [4]. Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer [5].

単位の定義

1ユニットは、pH 6.0、40°C、1分間で、Z-Arg-Arg 7-アミド-4-メチルクマリンから1 nmolの7-アミノ-4-メチルクマリンを遊離させる量です。

物理的形状

凍結乾燥品(リン酸バッファ-塩含有)

調製ノート

Dissolves in water at 1 mg/mL concentration, yieliding a clear solution.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable