This product is prepared in 50 mM sodium acetate buffer containing 1 mM EDTA, pH 5.0. This buffer may also be used to dilute the material further. This item has not been tested with other buffers as a diluent, however 25 mM MES has also been cited in the literature. See the link below to review this reference:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-cathepsin-b
219364
Cathepsin B, Human Liver
Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.
別名:
Cathepsin B, Human Liver
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About This Item
CAS番号:
MDL番号:
UNSPSCコード:
12352202
NACRES:
NA.77
現在、価格および在庫状況を閲覧できません。
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由来生物
human liver
品質水準
詳細
Merck USA index - 14, 1905
アッセイ
>95% (SDS-PAGE)
フォーム
liquid
比活性
≥50 units/mg protein
精製方法
affinity chromatography
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
テクニック
activity assay: suitable
pH範囲
3.5—6.0
適合性
suitable for molecular biology
アプリケーション
life science and biopharma
輸送温度
wet ice
保管温度
−70°C
遺伝子情報
human ... CTSB(1508)
詳細
Research area: Cell Signaling
Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase[1] and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.[2]
Optimal pH: 3.5-6.0.
Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase[1] and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.[2]
Optimal pH: 3.5-6.0.
アプリケーション
生物化学的／生理学的作用
Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins.[2] Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
単位の定義
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.
物理的形状
In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
調製ノート
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
再構成
Following initial thaw, aliquot and freeze (-70°C).
その他情報
Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
219364-5U:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
BinQing Wei et al.
Journal of medicinal chemistry, 61(3), 989-1000 (2017-12-12)
Antibody-drug conjugates (ADCs) have become an important therapeutic modality for oncology, with three approved by the FDA and over 60 others in clinical trials. Despite the progress, improvements in ADC therapeutic index are desired. Peptide-based ADC linkers that are cleaved
Lysosomal pathways to cell death and their therapeutic applications
Cesen MH, et al.
Experimental Cell Research, 1245-1251 (2012)
Lorna Kategaya et al.
Nature, 550(7677), 534-538 (2017-10-19)
The ubiquitin system regulates essential cellular processes in eukaryotes. Ubiquitin is ligated to substrate proteins as monomers or chains and the topology of ubiquitin modifications regulates substrate interactions with specific proteins. Thus ubiquitination directs a variety of substrate fates including
Vito Turk et al.
Biochimica et biophysica acta, 1824(1), 68-88 (2011-10-26)
It is more than 50 years since the lysosome was discovered. Since then its hydrolytic machinery, including proteases and other hydrolases, has been fairly well identified and characterized. Among these are the cysteine cathepsins, members of the family of papain-like
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We have bought this product (Cathepsin B, Human Liver, 219364), and the size is 5U. We are facing a problem with the working stock concentration. Do we need to resubstitute the volume into MQ water or buffer?
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