Volatile Fraction of Limoncello Using SPME on the SLB-5ms
Materiali
Colonna analitica
Fibra per SPME
Unità fibra SPME polidimetilsilossano (PDMS)
100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, plain red hubProdotti correlati
Standard
CONDITIONS
sample/matrix
Limoncello diluted 1:100 in water, then 5 mL of this solution transferred to a 10 mL crimped vial spiked with 100 μL of a 100 ppm solution of nonane (internal standard)
SPME fiber
100 μm polydimethylsiloxane (57300-U)
extraction
headspace, with a pre-equilibration period of 10 min. at 40 °C, and then a fiber exposure of 30 min. at 40 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 ppm
desorption process
250 °C for 5 min
column
SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven
40 °C, 3 °C/min to 250 °C (2 min)
inj. temp.
250 °C
detector
FID, 260 °C (sampling rate 80 ms)
carrier gas
helium, 35.0 cm/sec
injection
splitless
liner
0.75 mm I.D. SPME (2633901)
sample
Limoncello liquor
Descrizione
Risultati analitici
In this application, SPME was used for the analysis of volatiles in limoncello using a headspace method. SPME is ideal for the analysis of aroma volatiles in flavored beverages such as liquors. A 100 μm PDMS fiber was inserted into the headspace of the limoncello sample for 30 minutes at 40 °C followed by GC analysis using FID detection. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.
Note legali
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany