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NA2020

Sigma-Aldrich

GenElute™ Blood Genomic DNA Kit

sufficient for 350 purifications

Sinonimo/i:

Blood Genomic DNA, Gen Elute

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1 KIT
1.180,00 €

1.180,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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1 KIT
1.180,00 €

About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

1.180,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

impiego

sufficient for 350 purifications

Livello qualitativo

200

tecniche

DNA purification: suitable

Temperatura di conservazione

15-25°C
room temp

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Descrizione generale

The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Applicazioni

The purified genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning

Caratteristiche e vantaggi

  • Starting material: Up to 200 μl of fresh or aged blood
  • Expected yield: Up to 10 μg
  • Elution volume: 400 μl
  • Time required: <40 min
  • A260/A280 ratio: 1.6 - 1.9
  • Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate

Principio

The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.

The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Altre note

For additional information, please see www.sigma-aldrich.com/genomicdna.

Note legali

GenElute is a trademark of Sigma-Aldrich Co. LLC

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

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P2308

Proteinasi K, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Bromuro di etidio, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarosio, BioReagent, for molecular biology, low EEO

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tampone Tris-borato-EDTA, 5× Concentrate

D9780

Lambda DNA Hind III Digest, for DNA electrophoresis

R6148

RNase A solution

NA2010

GenElute™ Blood Genomic DNA Kit, sufficient for 70 purifications

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

Proteinasi K, Reagents designed and manufactured under current ISO 13485 certification.

Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Avvertenze

Danger

Indicazioni di pericolo

H290,H302,H315,H318,H334,H335,H410

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Elenchi normativi

Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.

EU REACH Annex XIV (Authorisation List)

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Certificati d'analisi (COA)

Lot/Batch Number
SLCL5325
SLCH7104
SLCF4757
SLCC2033
SLBZ2615

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Protocolli

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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