11814770001
Roche
DNA Isolation Kit for Cells and Tissues
sufficient for 10 isolation(s)
Sinonimo/i:
DNA isolation
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About This Item
Codice UNSPSC:
41105500
Prodotti consigliati
impiego
sufficient for 10 isolation(s)
Livello qualitativo
Produttore/marchio commerciale
Roche
Confezionamento
kit of for 10 isolations
Categorie correlate
Descrizione generale
The DNA Isolation Kit for Cells and Tissues provides medium- and large-scale preparation of purified genomic DNA ranging in size from 50 to 150 kb. Remove contaminating RNA and proteins from a wide variety of biological specimens (mammalian tissue, cultured cells, yeast, gram-negative bacteria or mouse tail).
Applicazioni
DNA Isolation Kit for Cells and Tissues has been used to isolate DNA from a wide variety of starting materials. The isolated DNA is suitable for many molecular biology applications:
- Genomic Southern blotting
- Sequencing
- Restriction digestion
- PCR/long PCR
- Cloning
Caratteristiche e vantaggi
Obtain increased yields of DNA in less than 2.5 hours.
Benefit from a simple, straightforward procedure (compared with column based methods).
Increase safety and convenience.
Eliminate the use of chaotropic salts, anion-exchange columns, and hazardous organic solvents.
Reduce purification time.
All required reagents are included in the kit.
Benefit from a simple, straightforward procedure (compared with column based methods).
Increase safety and convenience.
Eliminate the use of chaotropic salts, anion-exchange columns, and hazardous organic solvents.
Reduce purification time.
All required reagents are included in the kit.
Componenti
- Cellular Lysis Buffer
- Proteinase K Solution
- RNase Solution
- Protein Precipitation Solution
Qualità
Each lot of the DNA Isolation Kit for Cells and Tissues is tested for the absence of DNase contamination. Function tests to purify DNA from bovine liver, followed by specific amplification with the Expand High Fidelity PCR System were performed.
Nota sulla preparazione
Sample material is homogenized in Cellular Lysis Buffer in the presence of a strong anionic detergent and proteinase K. RNA is eliminated by RNase treatment and proteins are removed by selective precipitation and centrifugation. The purified DNA is finally recovered from solution by isopropanol precipitation.
Altre note
Figure 1: Amplification of both short and long DNA fragments from genomic DNA prepared with the DNA Isolation Kit for Cells and Tissues. Genomic DNA was isolated from a variety of sources and then amplified with either Taq DNA Polymerase, the Expand High Fidelity PCR System, or the Expand Long Template PCR System.
Lanes 2, 3: Human DMD fragment (268 bp) and mouse c-myc fragment (580 bp), amplified with Taq DNA Polymerase
Lanes 4, 5, 7, 8: Human c-myc fragment (1.2 kb), mouse β2-microglobulin fragment (3.6 kb), bovine lysozyme gene fragment (6.9 kb), and human tPA gene fragment (9.3 kb), all amplified with the Expand High Fidelity PCR System
Lanes 6, 9, 10: Mouse α-2 collagen gene fragments (5.6 kb and 10.4 kb) and human β-globin fragment (23 kb), amplified with the Expand Long Template PCR System
Lanes 1, 11 : DNA Molecular Weight Markers VI and II
Lanes 2, 3: Human DMD fragment (268 bp) and mouse c-myc fragment (580 bp), amplified with Taq DNA Polymerase
Lanes 4, 5, 7, 8: Human c-myc fragment (1.2 kb), mouse β2-microglobulin fragment (3.6 kb), bovine lysozyme gene fragment (6.9 kb), and human tPA gene fragment (9.3 kb), all amplified with the Expand High Fidelity PCR System
Lanes 6, 9, 10: Mouse α-2 collagen gene fragments (5.6 kb and 10.4 kb) and human β-globin fragment (23 kb), amplified with the Expand Long Template PCR System
Lanes 1, 11 : DNA Molecular Weight Markers VI and II
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9
Isolation of High Molecular Weight DNA
The kit simplifies the isolation of 50 to 150 kb genomic DNA.
Isolation of High Molecular Weight DNA
The kit simplifies the isolation of 50 to 150 kb genomic DNA.
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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