D9780

Lambda DNA Hind III Digest

for DNA electrophoresis

• Sinonimo/i: Hind III digested bacteria phage Lambda DNA
• Codice UNSPSC: 41141827
• NACRES: NA.25

Proprietà

• Grado: for molecular biology
• Forma fisica: liquid
• impiego: 100 uses (0.1 mg size); 1000 uses (1 mg size); 20 uses (20 μg size); 500 uses (0.5 mg size)
• Compatibilità: suitable for electrophoresis (DNA)
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Applicazioni

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componenti

Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Altre note

Contains 8 fragments, 125–23,130 bp

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Nota sulla preparazione

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable