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T9284

Sigma-Aldrich

Triton X-100

BioXtra

Sinonimo/i:

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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100 ML
175,00 €
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258,00 €
1 L
372,00 €

175,00 €


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100 ML
175,00 €
500 ML
258,00 €
1 L
372,00 €

About This Item

Formula condensata:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
Numero CAS:
Beilstein:
2315025
Numero MDL:
Codice UNSPSC:
12161900
ID PubChem:
NACRES:
NA.21

175,00 €


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Origine biologica

synthetic

Livello qualitativo

Descrizione

non-ionic

Nome Commerciale

BioXtra

Stato

liquid

tecniche

permeability assay: suitable

Impurezze

≤0.05% Phosphorus (P)

Residuo alla calcinazione

≤0.4%

Temp. transizione

cloud point 65 °C
pour point ~7 °C

Solubilità

water: soluble

Densità

1.065 g/cm3 at 20 °C (68 °F)

Anioni in tracce

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

Cationi in tracce

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.1%
Pb: ≤0.001%
Zn: ≤0.0005%

Stringa SMILE

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
HNLXNOZHXNSSPN-UHFFFAOYSA-N

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Descrizione generale

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]

Applicazioni

Triton X-100 has been used:
  • Along with Span® 80 and Tween® 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique[3][4][5]
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling[3]
  • In the blocking solution during immunocytochemistry procedure[6][7]
  • During deglycosylation procedure[8]
  • For membrane solubilization[9]
  • For cell permeabilization[9][10]
  • In decellularization of porcine heart valve tissue[11]

Azioni biochim/fisiol

Tensioattivo non ionico di uso comune adoperato per il recupero dei componenti di membrana in condizioni delicate non denaturanti.

Caratteristiche e vantaggi

  • BioXtra Grade product for your Cell Biology and Biochemical research
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

Altre note

For additional information on our range of Biochemicals, please complete this form.

Note legali

Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

483.8 °F - closed cup

Punto d’infiammabilità (°C)

251 °C - closed cup

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Elenchi normativi

Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XIV (Authorisation List)

CAS No.

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Certificati d'analisi (COA)

Lot/Batch Number

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Structural, metabolic and developmental evaluation of ovulated rabbit oocytes before and after cryopreservation by vitrification and slow freezing.
Theriogenology, 74(5), 847-855 (2010)
Membrane and acto-myosin tension promote clustering of adhesion proteins.
Delanoe-Ayari H, et al.
Proceedings of the National Academy of Sciences of the USA, 101(8), 2229-2234 (2004)
Detection of fetal DNA in maternal plasma by microarray coupled with emulsions PCR.
Ge Q, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 369(1), 82-88 (2006)
Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats.
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Proceedings of the National Academy of Sciences of the USA, 100(15), 8817-8822 (2003)

Protocolli

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

Domande

1–8 di 8 domande  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  3. Is this the Triton X-100 (T9284) considered sterile?

    1 risposta
    1. This product is not sterile. Should this be a requirement, see product 648463, a 10% sterile solution of Triton X-100. https://www.sigmaaldrich.com//product/mm/648463

      Utile?

  4. Hi, I wanna to ask how to make 0.1% triton. how much TritonX-100 I should use. Because I really can't understand what does X-100 mean.

    1 risposta
    1. This item is considered 100 % Triton X-100. A 1000 x dilution can be used to make a 0.1% triton solution.

      Utile?

  5. What's the surface tension of this product?

    1 risposta
    1. T9284 is Triton X100 that comes from Dow Chemical Company. The surface tension is 33 dynes/cm at 1% actives, 25°C as per the technical data sheet on the vendor's website: https://www.dow.com/en-us/pdp.triton-x-100-surfactant.85735z.html#tech-content

      Utile?

  6. What is the Surface Tension value of Triton X-100? and, what is the hydrophilic/lipophilic balance (HLB) and critical micelle concentration (cmc) of Triton X-100?

    1 risposta
    1. • Cloud Point @1% Aqueous (ASTM D 2024): 66 °C
      • CMC (25°C): 189 PPM
      • Foam Height - Initial (0.1 wt% actives): 128 mm
      • Foam Height-5 minutes (0.1 wt% actives): 107 mm
      • HLB: 13.4 Moles EO: 9.5 Pour Point: 1 °C
      • Surface Tension (1% actives, 25 °C): 33 dynes/cm

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  7. What is the expiration date for the products?

    1 risposta
    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 2.5 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Utile?

  8. Hello, I bought the following product: Triton X-100 (T9284) - BioXtra and I was wondering what grade is it? Is it suitable for nucleic acid extraction? Thanks, Irene

    1 risposta
    1. The BioXtra products are characterized by more stringent testing protocols that may include trace metal analysis and specialized suitability testing. This product is not tested specifically for nucleic acid extraction. However, there is no specific aspect of the product that would render it unsuited for this application. For future reference, the molecular biology grade (T8787) may be of interest. This product is tested for DNAse and RNAse activity. See the links below to review this product option:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8787-BULKSLCJ6163.pdf
      https://www.sigmaaldrich.com/product/sigma/t8787

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