814449

TSKgel^® Boronate-5PW (10 µm) HPLC Columns

L x I.D. 5 cm × 5 mm, m-aminophenyl boronate phase

• Szinonimák: TSKgel^® Boronate-5PW HPLC Column
• UNSPSC kód: 41115711

Tulajdonságok

• product name: TSKgel^® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
• material: glass
• termékcsalád: TSKgel^®
• analit kémiai osztály(ok): proteins
• gyártó/kereskedő neve: Tosoh 14449
• parameter: ≤20% organic solvent; ≤3 M salt concentration; >0 °C temperature; 1.2 mL/min flow rate; 30 bar max. pressure
• technika/technikák: HPLC: suitable
• H × belső átmérő: 5 cm × 5 mm
• matrix: hydroxylated methacrylic polymer
• mátrix aktív csoport: m-aminophenyl boronate phase
• részecskeméret: 10 μm
• működési pH: 2-9
• kapacitás: 40 μmol/mL, resin adsorption capacity (sorbitol)
• elválasztástechnika: affinity
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

Javasolt termékek

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Jogi információk

TSKgel is a registered trademark of Tosoh Corporation