T9033

Thapsigargin

≥98% (HPLC), solid film

• Szinonimák: (-)-Thapsigargin, TG1
• Tapasztalati képlet (Hill-képlet): C₃₄H₅₀O₁₂
• CAS-szám: 67526-95-8
• Molekulatömeg: 650.75
• MDL-szám: MFCD00083511
• UNSPSC kód: 12352200
• PubChem Substance ID: 24278762
• NACRES: NA.77

Tulajdonságok

• biológiai forrás: plant (Thapsia garganica)
• Minőségi szint: 300
• Teszt: ≥98% (HPLC)
• Forma: solid film
• oldhatóság: acetonitrile: soluble 9.80-10.20 mg/mL; DMSO: soluble; ethanol: soluble
• tárolási hőmérséklet: −20°C
• SMILES string: [H][C@@]12C([C@H](OC([C@]3(O)C)=O)[C@]3(O)[C@@H](OC(CCC)=O)C[C@]2(C)OC(C)=O)=C(C)[C@H](OC(/C(C)=C(C)/[H])=O)[C@H]1OC(CCCCCCC)=O
• InChI: 1S/C34H50O12/c1-9-12-13-14-15-17-24(37)43-28-26-25(20(5)27(28)44-30(38)19(4)11-3)29-34(41,33(8,40)31(39)45-29)22(42-23(36)16-10-2)18-32(26,7)46-21(6)35/h11,22,26-29,40-41H,9-10,12-18H2,1-8H3/b19-11-/t22-,26+,27-,28-,29-,32-,33+,34+/m0/s1
• Nemzetközi kémiai azonosító kulcs: IXFPJGBNCFXKPI-FSIHEZPISA-N

Leírás

Általános leírás

Thapsigargin, a guaianolide-type sesquiterpene lactone, is a widely used inhibitor of the ubiquitous sarcoplasmic/endoplasmic reticulum Ca²⁺ ATPase (SERCA). It can activate endoplasmic reticulum (ER) stress and the unfolded protein response (UPR). Thapsigargin is a natural phytochemical found in the roots and fruits of Mediterranean plants from Thapsia garganica (Apiaceae) species. Thapsigargin promotes the release of translational arrest and induces proteome-wide changes in metabolic pathways within cells infected with coronavirus (CoV).

Alkalmazás

Thapsigargin has been used as an endoplasmic reticulum (ER) stress inducer:

• to study its effect on cyclase-associated protein 2 (CAP2) expression in liver cancer cells
• in the cell viability experiment using ARPE -19 cells (human retinal pigment epithelial cells)
• to study its effect on lipocalin 2 (LCN2) glycosylation in primary hepatocytes
• to study the consequences of calcium depletion in the endoplasmic reticulum (ER) of cells
• to study its effect on tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA expression in rat Sertoli cells

Biokémiai/fiziológiai hatások

Thapsigargin is a potent, cell-permeable, IP₃-independent intracellular calcium release. It blocks the transient increase in intracellular Ca²⁺ induced by angiostatin and endostatin. It also induces apoptosis by disrupting intracellular free Ca²⁺ levels and is incorporated into chemotherapeutic prodrug formulations.

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Biztonsági adatok

• Piktogramok: GHS08,GHS07
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315,H319,H334,H335
• Óvintézkedésre vonatkozó mondatok: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Veszélyességi osztályok: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Célzott szervek: Respiratory system
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Faceshields, Gloves