Összes fotó(4)
Fontos dokumentumok
T7539
Tumor Necrosis Factor-α from mouse
≥95%, recombinant, expressed in E. coli, powder, suitable for cell culture
Szinonimák:
mTNF-α, TNF-α
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(4)
About This Item
Javasolt termékek
Terméknév
Tumor Necrosis Factor-α from mouse, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
biológiai forrás
mouse
Minőségi szint
rekombináns
expressed in E. coli
Teszt
≥95%
Forma
powder
hatékonyság
0.01-0.5 ng/mL EC50
molekulatömeg
17 kDa
kiszerelés
pkg of 10 μg
pkg of 50 μg
tárolási körülmény
avoid repeated freeze/thaw cycles
technika/technikák
cell culture | mammalian: suitable
szennyeződések
≤1 EU/μg protein endotoxin
szín
white
oldhatóság
water: soluble
UniProt elérési szám
tárolási hőmérséklet
−20°C
Géninformáció
mouse ... Tnf(21926)
Alkalmazás
Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
- to activate MS1 endothelium cells
- to induce lipolysis in adipocytes
- to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation
Biokémiai/fiziológiai hatások
Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.
Egység definíció
One unit is the amount required to induce half-maximal cytolysis of L929 cells.
Fizikai forma
Lyophilized from 10 mM sodium phosphate, pH 7.5
Egyéb megjegyzések
Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Irrit. 2 - Skin Irrit. 2
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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Glucocorticoid-independent repression of tumor necrosis factor (TNF) $\alpha$-stimulated interleukin (IL)-6 expression by the glucocorticoid receptor a potential mechanism for protection against an excessive inflammatory response
Verhoog NJD, et al.
The Journal of Biological Chemistry, 286(22), 19297-19310 (2011)
SDF-1--CXCR4 differentially regulates autoimmune diabetogenic T cell adhesion through ROBO1--SLIT2 interactions in mice
Glawe JD, et al.
Diabetologia, 56(10), 2222-2230 (2013)
Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-$\alpha$ (TNF-$\alpha$)-induced lipolysis in adipocytes
Yang X, et al.
The Journal of Biological Chemistry, 286(47), 40477-40485 (2011)
Fen Li et al.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 40(9), 945-954 (2015-09-27)
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Fabrícia Lima Fontes et al.
BMC medical genetics, 16, 70-70 (2015-09-01)
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Lipid Induced Insulin Resistance
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