Ugrás a tartalomra
Merck

T4528

Sigma-Aldrich

Thioglucosidase from Sinapis alba (white mustard) seed

≥100 units/g solid

Szinonimák:

Glucosinolase, Myrosinase, Thioglucoside glucohydrolase

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

CAS-szám:
Enzyme Commission szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.54

biológiai forrás

plant seeds (Sinapis alba)

Minőségi szint

Forma

solid

specifikus aktivitás

≥100 units/g solid

tárolási hőmérséklet

−20°C

Általános leírás

Thioglucosidase, also called as myrosinase, is present in the myrosin cells that do not contain glucosinolates. This enzyme is obtained from several plant sources, such as Lepidium sativum, L. Sinapis alba and Brassica napus.

Alkalmazás

Thioglucosidase from Sinapis alba (white mustard) seeds has been used as a standard to quantify myrosinase activity and in column glucosinolate analysis of plant samples.
Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content. Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana.

Biokémiai/fiziológiai hatások

Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits.
Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties.

Egység definíció

One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C.

Piktogramok

Health hazard

Figyelmeztetés

Danger

Figyelmeztető mondatok

Óvintézkedésre vonatkozó mondatok

Veszélyességi osztályok

Resp. Sens. 1

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


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Analitikai tanúsítványok (COA)

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Dokumentumtár megtekintése

George F Antonious et al.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 44(3), 311-316 (2009-03-13)
Glucosinolates (GSLs), a group of compounds found in Brassica plants, are toxic to some soil-borne plant pathogens because of the toxicity of their hydrolysis products, isothiocyanates. Other phytochemicals found in Brassica plants, such as phenols and ascorbic acid, may compliment
Shikha Saha et al.
Molecular nutrition & food research, 56(12), 1906-1916 (2012-10-31)
Sulforaphane (a potent anticarcinogenic isothiocyanate derived from glucoraphanin) is widely considered responsible for the protective effects of broccoli consumption. Broccoli is typically purchased fresh or frozen and cooked before consumption. We compared the bioavailability and metabolism of sulforaphane from portions
Niels Agerbirk et al.
Phytochemistry, 153, 79-93 (2018-06-11)
Glucosinolates are found in plants of the order Brassicales and hydrolyzed to different breakdown products, particularly after tissue damage. In Barbarea vulgaris R.Br. (Brassicaceae), the dominant glucosinolate in the investigated "G-type" is glucobarbarin, (S)-2-hydroxy-2-phenylethylglucosinolate. Formation of the nitrile from glucobarbarin
Wei Chen et al.
Frontiers in physiology, 13, 1013092-1013092 (2022-11-08)
Glycoside hydrolase family 1 (GH1) members exhibit a broad substrate spectrum and play important roles in insect-plant interactions, such as the defensive β-glucosidase and β-thioglucosidase (so-called myrosinase). However, knowledge about the expression profiling and function of glycoside hydrolase family 1
Teresa Oliviero et al.
Molecular nutrition & food research, 62(18), e1700837-e1700837 (2018-03-14)
Optimization of bioavailability of dietary bioactive health-beneficial compounds is as important as increasing their concentration in foods. The aim of this study is to explore the change in bioavailability of isothiocyanates (ITCs) in broccoli sprouts incorporated in protein, fiber, and

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