T1378

Sigma 7-9^®

≥99% (titration), crystalline, Sigma 7-9^®

• Szinonimák: Trizma^® base, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Lineáris képlet: NH₂C(CH₂OH)₃
• CAS-szám: 77-86-1
• Molekulatömeg: 121.14
• Beilstein: 741883
• EC-szám: 201-064-4
• MDL-szám: MFCD00004679
• UNSPSC kód: 12352104
• PubChem Substance ID: 24899961
• NACRES: NA.25

Tulajdonságok

• product name: Sigma 7-9^®, ≥99% (titration), crystalline
• leírás: aminopeptidase substrate
• Minőségi szint: 200
• Teszt: ≥99% (titration)
• form: crystalline
• tárolási körülmény: dry at room temperature
• technika/technikák: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable
• szennyeződések: ≤0.5% water (Karl Fischer)
• szín: white to off-white
• pH: 10.5-12
• hasznos pH-tartomány: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• oldhatóság: water: 0.667 g/mL, clear, colorless to faintly yellow
• absorption: ≤0.4 at 290 at 40%
• alkalmazás(ok): clinical research; diagnostic assay manufacturing; life science and biopharma; sample preparation
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• Nemzetközi kémiai azonosító kulcs: LENZDBCJOHFCAS-UHFFFAOYSA-N

Leírás

Általános leírás

Sigma 7-9^® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.

Alkalmazás

Sigma 7-9^® has been used:

• as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)
• as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence

Tulajdonságok és előnyök

• Suitable for Biological and Biochemical Research
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

Egyéb megjegyzések

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

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Jogi információk

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Gloves