SRP6455

EpCAM/CD326 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Szinonimák: DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK1, TACSTD1, TROP1
• UNSPSC kód: 12352200
• NACRES: NA.32

Tulajdonságok

• biológiai forrás: human
• rekombináns: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Teszt: ≥95% (SDS-PAGE)
• Forma: lyophilized
• molekulatömeg: calculated mol wt 29 kDa (after removal of signal peptide.); observed mol wt 33-36 kDa by SDS-PAGE
• kiszerelés: pkg of 10 and 50 μg
• szennyeződések: <1 EU/μg endotoxin (LAL test)
• UniProt elérési szám: P16422
• kiszállítva: wet ice
• tárolási hőmérséklet: −20°C
• Géninformáció: human ... EPCAM(4072)

Leírás

Általános leírás

DTT-reduced. rh EpCAMProtein migrates as poly peptide due to glycosylation. Gln24 is the predicted N-terminal.

Epithelial cell adhesion molecule (EpCAM) is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A (mAb) antigen. The gene encoding EpCAM is localized on human chromosome 2p21.

Biokémiai/fiziológiai hatások

Epithelial cell adhesion molecule (EpCAM) is intricately linked with the cadherin-catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The protein has several cellular functions, like epithelial-specific cell-adhesion, cell signaling, migration, proliferation and differentiation. It is expressed in most epithelial malignancies (like epithelial ovarian cancer) and was reported as a tumor marker or a candidate of molecular targeting therapy. EpCAM cross signaling with N-cadherin involves phosphoinositide-3-kinase (Pi3K), resulting in the abrogation of the cadherin adhesion complexes in epithelial cells.

Fizikai forma

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Feloldás

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable