SRE0006
Thymidine Phosphorylase, recombinant from Escherichia coli
recombinant, expressed in E. coli, Suitable for manufacturing of diagnostic kits and reagents, buffered aqueous solution, ≥500 units/mL
Szinonimák:
Gliostatins, PD-ECGF, Thymidine:orthophosphate deoxy-D-ribosyltransferase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
CAS-szám:
MDL-szám:
UNSPSC kód:
12352204
NACRES:
NA.54
Javasolt termékek
rekombináns
expressed in E. coli
Minőségi szint
form
buffered aqueous solution
koncentráció
≥500 units/mL
technika/technikák
inhibition assay: suitable
szín
colorless to yellow
oldhatóság
soluble
water: soluble
NCBI elérési szám
UniProt elérési szám
alkalmazás(ok)
diagnostic assay manufacturing
kiszállítva
wet ice
tárolási hőmérséklet
2-8°C
Géninformáció
Escherichia coli ... deoA(948901)
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Általános leírás
Research area: CELL SIGNALING
The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which is identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which is identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
Alkalmazás
Thymidine phosphorylase has been used:
- in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy.
- in a study to investigate implications for the clinical efficacy of nucleoside analogues.
Biokémiai/fiziológiai hatások
An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
Thymidine phosphorylase catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the thymidine salvage pathway and pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The enzyme inhibits apoptosis and induces angiogenesis thereby promoting tumor growth and metastatic process. Moreover, thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.
Egység definíció
One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.
Elkészítési megjegyzés
Cloned from E. coli and produced in overexpressing E. coli
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
The dual role of thymidine phosphorylase in cancer development and chemotherapy
Bronckaers A, et al.
Medicinal Research Reviews, 29(6), 903-953 (2009)
Thymidine kinase 1 and thymidine phosphorylase expression in non-small-cell lung carcinoma in relation to angiogenesis and proliferation
Brockenbrough J S, et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 57(11), 1087-1097 (2009)
Structures of native human thymidine phosphorylase and in complex with 5-iodouracil
Mitsiki E, et al.
Biochemical and Biophysical Research Communications, 386(4), 666-670 (2009)
Sheng-hua Zhang et al.
Cancer chemotherapy and pharmacology, 72(4), 777-788 (2013-08-27)
Capecitabine (CAP), a prodrug, needs to be converted to 5-fluorouracil by several key enzymes, including thymidine phosphorylase (TP). To improve the therapeutic index, potentiation of antitumor activity of CAP is required. In this study, we explored whether lidamycin (LDM), an
Akihiko Hatano et al.
Organic & biomolecular chemistry, 11(40), 6900-6905 (2013-09-24)
Herein, we describe β-selective coupling between a modified uracil and a deoxyribose to produce functionalized nucleosides catalyzed by thymidine phosphorylase derived from Escherichia coli. This enzyme mediates nucleobase-exchange reactions to convert unnatural nucleosides possessing a large functional group such as
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