SAE0003

4-Hydroxyphenylpyruvate Dioxygenase from Streptomyces avermitilis

recombinant, expressed in E. coli

• Enzyme Commission szám: 1.13.11.27 (BRENDA, IUBMB)
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• rekombináns: expressed in E. coli
• Minőségi szint: 200
• Forma: liquid
• specifikus aktivitás: ≥5 U/mg
• molekulatömeg: ~41.8 kDa
• kiszállítva: dry ice
• tárolási hőmérséklet: −70°C

Leírás

Általános leírás

4-Hydroxyphenylpyruvate Dioxygenase (4-HPPD) is a key enzyme in the catabolism of tyrosine and phenylalanine. 4-HPPD catalyzes the synthesis of homogenistate (2,5-dihydroxyphenylacetate) from ρ-hydroxyphenylpyruvate and molecular oxygen. 4-HPPD also catalyzes the conversion of α-ketoisocaproate to Β-hydroxyisovalerate. 4-HPPD requires Fe²⁺ as an essential cofactor.

Rat 4-HPPD has a minimum of 80% homology with human 4-HPPD. Rat 4-HPPD is expressed abundantly in hepatocytes. It is also expressed in kidney, particularly in epithelial cells of the proximal tubules and the distant tubules. 4-HPPD has been identified as a potential biomarker of drug-induced liver injury in rat studies.

Egység definíció

One unit will oxidize 1 μmole of molecular oxygen per minute at pH 7.0, 25 °C, in the presence of 4-hydroxyphenylpyruvate.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable