SAB5200017

Monoclonal Anti-HIF-1 α antibody produced in mouse

clone ESEE122, 1 mg/mL, purified immunoglobulin

• Szinonimák: Anti-ARNT interacting protein, Anti-HIF1 alpha, ESEE122, Anti-HIF1A, Anti-Hypoxia inducible factor 1 alpha, Anti-MOP1, Anti-PASD8
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ELISA (i); ICC; IF; IHC; WB
• klón: ESEE122, monoclonal
• faj reaktivitás: bovine, mouse, human, rat
• citations: 11
• technika/technikák: immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: suitable; western blot: suitable

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 100
• konjugátum: unconjugated
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: ESEE122, monoclonal
• Forma: buffered aqueous glycerol solution
• faj reaktivitás: bovine, mouse, human, rat
• koncentráció: 1 mg/mL
• technika/technikák: immunocytochemistry: suitable; immunohistochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: suitable; western blot: suitable
• izotípus: IgG1
• NCBI elérési szám: NP_034561.2
• UniProt elérési szám: Q16665
• kiszállítva: wet ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: mouse ... Hif1a(15251)

Leírás

Általános leírás

Hypoxia-inducible factor-1 α (HIF1 α) gene encodes the α subunit of HIF-1. HIF-1α contains oxygen-dependent degradation (ODD) domain that mediates the interaction between HIF-1α and the E3 ubiquitin ligase complex, which in turn facilitates continuous poly ubiquitination of HIF-1α in normoxic cells. The gene encoding it is localized on human chromosome 14q23.2. HIF1 α belongs to the basic helix-loop-helix family of transcription factors.

Egyediség

Specific for HIF1-alpha.

Immunogén

Recombinant fragment corresponding to amino acids 329-530

Alkalmazás

Monoclonal Anti-HIF-1 α antibody produced in mouse has been used in siRNA assay and western blot analysis.

Biokémiai/fiziológiai hatások

Hypoxia-inducible factor-1(HIF1) plays an essential role in cellular and systemic homeostatic responses to hypoxia. During hypoxia, the two subunits of this factor undergo post-translational modifications which in turn promote transactivation. HIF-1α gene loss is associated with development of nonpapillary renal carcinomas. The encoded protein functions as a key regulator of the vascular endothelial growth factor (VEGF) under hypoxic response in human stromal cells in vitro. HIF-1α protein expression is often seen in invasive breast cancer. Experimental data demonstrates that downregulation of HIF-1α decreases tumorigenicity of MCF-7 (human breast adenocarcinoma cell line) cells and propose a promising combination of both anti-HIF-1 strategy and traditional chemotherapy to improve cancer treatment.

Tulajdonságok és előnyök

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Fizikai forma

Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable